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纳米级碳量子点激活抗病毒免疫反应,并通过诱导差异替代拼接来调节异质核核核核蛋白接口

Tahir Farooq¹, Muhammad Dilshad Hussain², Dandan Liu³

¹Plant Protection Research Institute and Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, PR China.

Pesticide biochemistry and physiology

|June 11, 2025

概括

碳量子点 (CQD) 通过重新编程基因表达和替代拼接来增强植物对病毒的免疫力. 这项研究揭示了CQD在提高植物弹性方面的潜力,并为作物保护提供了可持续的解决方案.

科学领域:

植物病理学植物病理学
纳米技术纳米技术
分子生物学分子生物学

背景情况:

植物病毒对全球粮食安全构成重大威胁.
可持续的抗病毒策略对于作物保护至关重要.
碳量子点 (CQD) 对抗病毒疗法有希望,但它们在植物免疫中的机制需要阐明.

研究的目的:

为了全面地绘制CQDs调制的抗病毒反应的转录重编程.
调查CQD,替代拼接 (AS) 事件和植物免疫之间的关联.
为了阐明CQDs对Nicotiana benthamiana的棉花叶卷曲穆尔坦病毒 (CLCuMuV) 的抗病毒机制.

主要方法:

用CQDs治疗的CLCuMuV感染的尼古提亚班塔米亚的转录组分析.
对替代拼接 (AS) 模式的分析,包括外子跳转和内子保留.
结构建模研究CQD与RNA结合蛋白的相互作用.

主要成果:

在注射后5天 (dpi),CQD上调了抗病毒免疫基因 (CaMK,WRKY,MAPK,STPK) 和应激反应途径.
转录反应转向减少20dpi的病毒引起的压力.
CQDs调节了AS模式,增加了外跳跃和内保留,特别是在感染后期.
CQDs诱导了hnRNP的构造变化,增强了它的结合能力.

结论:

CQDs通过基因表达调节和差异性AS事件动态调节植物抗病毒免疫反应.
通过减轻N. benthamiana的症状,CQD显示出针对CLCuMuV的显著抗病毒潜力.
CQD是提高植物抗病毒病原体的有效工具,为可持续的作物保护铺平了道路.

关键词:

另一个替代拼接.抗病毒免疫抗病毒免疫在CLCuMuVV中.纳米颗粒纳米颗粒工厂防御系统的工厂防御系统在 hnRNP 中.

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