基因组洞察力对抗万科米和提基基林耐药的Enterococcus faecium ST117的传播
Marie Brajerova1, Otakar Nyc1, Pavel Drevinek1
1Department of Medical Microbiology, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czech Republic.
Annals of clinical microbiology and antimicrobials
|June 11, 2025
概括
这项研究确定了在捷克的一家医院传播的抗万科米辛和tigecycline Enterococcus faecium ST117的传播. 结合分子和表型数据对于有效监测抗菌素耐药性至关重要.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 流行病学 流行病学
背景情况:
- 越来越多的抗胺素肠球菌 (VRE) 的发生率需要了解耐药性机制.
- 对VRE感染的有限治疗选择强调了流行病学监测的必要性.
研究的目的:
- 调查抗万科米辛和蒂吉环素耐药性肠球菌的流行病学.
- 使用全基因组测序 (WGS) 进行详细的分子表征.
主要方法:
- 从一家三级医院收集了102个VRE分离物 (2021年4月至12月).
- 在40个选定的隔离物中进行了抗菌敏感性测试和WGS (Illumina和MinION).
主要成果:
- 通过塑体上的vanA操作子和染色体上的vanB操作子识别了万科米辛耐药性.
- 通过tet(L),tet(M) 和rpsJ突变/删除检测到tigecycline耐药性.
- 观察到基因型和表型对达普素,三甲烯/硫甲醇,尼特洛黄因和四环素的耐药性之间的差异.
- 全基因组MLST揭示了克隆集群,其中ST117在Enterococcus faecium分离物中占主导地位 (80%).
结论:
- 证实了Enterococcus faecium ST117的传播,该病毒对万科米辛和提格环素具有耐药性.
- 强调综合分子和表型监测对于抗菌素耐药性监测的重要性.
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