在SHBG测定中的可变性及其对自由的计算估计的影响
Joeri Walravens1, Joanne Adaway2, Tim Reyns3
1Department of Internal Medicine and Pediatrics, Ghent University & Department of Endocrinology, University Hospital Ghent, Ghent, Belgium.
Annals of clinical biochemistry
|June 12, 2025
概括
对性激素结合球蛋白 (SHBG) 的血清测定显示出极小的差异,对计算自由 (cFT) 的影响很小. 然而,实验室的变异性和配方选择可以显著改变cFT结果和诊断.
科学领域:
- 内分泌学 在内分泌学.
- 临床化学 临床化学
- 实验室医学 实验室医学
背景情况:
- 计算的自由丸激素 (cFT) 对于评估丸激素暴露至关重要,它依赖于性激素结合球蛋白 (SHBG) 的测量.
- 在SHBG测定中的变化和实验室间的差异可能会影响cFT计算的准确性.
研究的目的:
- 评估不同SHBG测定选择和模拟的实验室间变异性对cFT水平的影响.
- 评估这些变异如何影响低阴性恋和高雄性恋的诊断.
主要方法:
- 来自113名男性和106名女性的血清样本使用三个SHBG免疫测试 (罗氏,阿博特,西门子) 进行了分析.
- 测试用LC-MS/MS进行测量;使用多种公式计算cFT并直接测量.
- 模拟了15%的实验室间SHBG变异性,以重新计算cFT并比较诊断比例.
主要成果:
- SHBG测定显示了良好的合规性,Abbott和西门子之间的最大差异为7%.
- 测试之间cFT水平的差异很小 (≤3%).
- 实验室间的变异性显著影响了诊断比例,这取决于所使用的计算公式.
结论:
- 选择SHBG测定对cFT水平和临床决策的影响有限.
- 实验室间的SHBG测量变化和cFT计算公式的选择可以显著影响结果和解释.
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