相关实验视频
Updated: Jun 13, 2025
08:23
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
Published on: November 1, 2024
543
RNAS-sgRNA:循环神经架构 在单向导RNA中寻找检测目标效应的搜索
1Department of Computer Science and Engineering, Islamic University of Science and Technology, Pulwama, India.
概括
RNA-sgRNA是一种新的混合模型,通过使用自动神经架构搜索和循环神经网络准确预测单向导RNA疗效,增强了CRISPR/Cas9基因组编辑. 这种工具可以提高基因编辑精度和效率,跨越各种细胞系.
科学领域:
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
- 计算生物学 计算生物学
背景情况:
- CRISPR/Cas9是一种强大的基因组编辑工具.
- 单向导RNA (sgRNA) 的效率差异很大,限制了CRISPR/Cas9的有效性.
- 准确预测sgRNA的目标有效性对于成功的基因编辑至关重要.
研究的目的:
- 开发一个自动化模型来评估CRISPR/Cas9 sgRNA在目标上的疗效.
- 为了提高sgRNA序列分类的准确性和效率.
- 提供一种增强个性化医学和遗传研究的工具.
主要方法:
- 将神经架构搜索 (NAS) 与循环神经网络 (RNN) 集成,以创建RNAS-sgRNA模型.
- 针对优化RNN结构的自动架构发现.
- 分析以二进制矩阵表示的sgRNA序列,以生成分类得分.
主要成果:
- 在多个细胞系 (HCT116,HEK293T,HeLa,HL60) 中,RNAS-sgRNA表现出显著的性能提升.
- 与CRISPRpred(SEQ) 和DeepCRISPR模型相比,在接收器操作特征曲线 (AUROC) 下面的面积取得了显著的改进.
- 通过转移学习,在较小的数据集上表现出卓越的性能,AUROC总体改进率为13.46%.
结论:
- RNAS-sgRNA提供了一种新的,自动化的方法来评估CRISPR/Cas9 sgRNA的疗效.
- 该模型显著优于现有方法,推进基因组编辑研究.
- 它利用转移学习的能力使它成为个性化医学和遗传应用的宝贵工具.
相关概念视频
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
02:30siRNA - Small Interfering RNAs
16.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.6K