相关实验视频

Updated: May 11, 2026

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Published on: February 20, 2019

单细胞多模式剖析揭示了一种新的CD26⁺ 纤维细胞亚群在动脉样硬化

Alexander C Bashore¹, Johana Coronel², Chenyi Xue²

¹Cardiovascular Research Institute, Icahn School of Medicine at Mount Sinai, New York, NY (A.C.B.).

Arteriosclerosis, thrombosis, and vascular biology

|June 12, 2025

概括

纤维细胞在动脉样硬化中发挥着关键作用. 研究人员确定了 CD26+ 纤维细胞,这些纤维细胞迁移到斑块纤维状盖中,可能稳定动脉样硬化病变.

科学领域:

心血管生物学心血管生物学
细胞和分子医学细胞和分子医学
免疫学免疫学免疫学

背景情况:

动脉样硬化病原体涉及复杂的细胞相互作用,纤维细胞未被充分探索.
了解纤维细胞异质性对于阐明它们在斑块发育和稳定中的作用至关重要.

研究的目的:

使用小鼠模型全面描述动脉样硬化中的纤维细胞多样性.
通过疾病进展的不同阶段来表征纤维细胞表型.

主要方法:

通过测序 (CITE-seq) 来利用转录组和表位组的细胞索引进行多原子分析.
在26周的时间里,在小鼠中采用了动脉样硬化模型的动脉样性饮食.
应用流细胞计和免疫组织化学用于细胞识别和定位.

主要成果:

确定了4个不同的纤维细胞亚群,包括一个类似于肌纤维细胞的亚群.
发现CD26作为纤维细胞亚群的特定标志物.
观察到CD26+纤维细胞从冒险细胞迁移到亲密细胞,在疾病进展过程中定位在纤维状盖内.

结论:

纤维细胞群体表现出表型多样性和动态变化在动脉样硬化.
CD26+纤维细胞代表一个独特的亚群,在动脉样硬化病变中扩大.
这些CD26+纤维细胞通过迁移到纤维状盖上,可能有助于斑块的稳定性.

关键词:

动脉动脉动脉动脉疾病的进展疾病的进展纤维细胞的细胞.现象型现象型是一种现象型.斑块,动脉样硬化,动脉样硬化

更多相关视频

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Published on: March 12, 2020

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Published on: October 4, 2021

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...