相关实验视频

Updated: May 8, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

在便样本中检测Streptococcus anginosus,使用PCR-CRISPR/Cas12a系统检测

Yan Zheng¹, BiXia Liu², QianFei Zuo³

¹Department of Gastroenterology, Chongqing Key Laboratory of Digestive Malignancies, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, PR China.

|June 12, 2025

概括

一种新的PCR-CRISPR/Cas12a方法可以在便中准确检测Streptococcus anginosus. 这种敏感的技术在超过一半的普通人群中发现了S. anginosus,这表明它在胃肠道瘤中的潜在作用.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

性链球菌 (S. anginosus) 是一种与胃肠道病理有潜在联系的细菌.
准确和敏感的检测方法对于了解其流行率和疾病中的作用至关重要.

研究的目的:

开发一种高度敏感和特定的PCR-CRISPR/Cas12a试验,用于检测肠杆菌在便样本中的存在.
评估一般人群中S. anginosus的流行情况.
探索其作为胃肠道瘤生物标志物的潜力.

主要方法:

设计的特定原料和crRNA针对S. anginosus的16SrDNA.
构建了一个PCR-CRISPR/Cas12a检测系统.
分析了来自普通人口的230个便样本的细菌DNA.

主要成果:

该PCR-CRISPR/Cas12a系统表现出高特异性和稳定性.
达到了10^-7 ng/μL的最小检测极限.
在一般人口51.7%的便样本中检测到S. anginosus.

结论:

开发的PCR-CRISPR/Cas12a测定是一种有效的工具,用于检测便中的S. anginosus.
这种方法为调查S. anginosus和胃肠道瘤之间的关联提供了可靠的基础.

关键词:

16S rDNA 是一个16S rDNA.这就是CRISPR/Cas12a.这种病原菌是Streptococcus anginosusus.诊断诊断诊断诊断诊断诊断便便便便

更多相关视频

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...