孕期慢性间歇性缺氧引发母体炎症并破坏胎盘应激反应
bioRxiv : the preprint server for biology
|June 12, 2025
概括
怀孕期间的慢性间歇性缺氧会改变母亲的炎症和细胞外囊泡,降低胎盘效率,而不会影响大鼠的胎儿生长.
科学领域:
- 生殖生物学 生殖生物学
- 产周医学 产周医学
- 母胎界面 母胎界面
背景情况:
- 孕期缺氧可以诱导胎盘氧化应激和炎症.
- 在细胞外囊泡 (EVs) 中循环的无细胞线粒体DNA (ccf-mtDNA) 可以促进炎症.
- 慢性间歇性缺氧 (CIH) 可能会影响母亲的炎症和胎盘功能.
研究的目的:
- 研究CIH对孕鼠母体炎症,循环EV和胎盘应激反应的影响.
- 为了确定CIH是否会改变母亲的炎症特征,EV特征以及胎盘抗氧化和自路径.
主要方法:
- 怀孕的老鼠从15-20个妊娠日开始暴露于CIH或normoxia.
- 使用qRT-qPCR进行量化ccf-mtDNA和EV相关的mtDNA (EV-mtDNA).
- 测量了与应激反应相关的母细胞因子,氧化应激 (AOPP) 和胎盘基因/蛋白质表达.
主要成果:
- CIH增加了胎盘体重和降低了胎盘效率,但没有影响胎儿生物识别.
- EV度下降,这表明每个EV的EV-mtDNA增加.
- 母体IL-18增加,胎盘抗氧化基因表达 (catalase, sod2) 和自蛋白 (Beclin-1, p62) 的上调.
结论:
- 孕期CIH破坏了母亲的细胞外囊形状和炎症标志物.
- CIH降低了胎盘效率,并调节了胎盘抗氧化和自机制.
- 在大鼠模型中,这些胎盘适应发生在不影响胎儿生长的情况下.
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