相关实验视频

Updated: Jun 13, 2025

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Published on: December 23, 2022

斯芬哥辛-1-酸盐通路在人类妊娠组织中被差异激活

Magdaleena Naemi Mbadhi, Hideji Fujiwara, Ruth Gill

bioRxiv : the preprint server for biology

|June 12, 2025

概括

在怀孕组织中,sphingosine-1-phosphate (S1P) 代谢和酶的调节方式不同. 在分娩期间肌子膜S1P增加表明肌子膜收缩性起作用.

科学领域:

产科和妇科产科和妇科
生物化学生物化学
生殖生物学生殖生物学

背景情况:

失调的肌体收缩性与产科并发症有关.
氨酸-1-酸盐 (S1P) 是妊娠组织中的炎症调节剂,但其代谢动态尚不清楚.
了解S1P代谢对于解决妊娠并发症至关重要.

研究的目的:

在人类孕期组织中对神素-1-酸盐 (S1P) 代谢酶和受体表达进行分析.
量化怀孕不同阶段的脂代谢.
调查S1P在肌肉膜收缩性中的作用.

主要方法:

从没有分娩的期末,有分娩的期末和没有分娩的早产期怀孕中收集了人类的肌子膜, decidua parietalis 和chorioamnion.
通过定量聚合酶链反应评估S1P代谢酶和受体的信使RNA (mRNA) 表达.
量化了使用向液体染色学-并联质谱的脂蛋白.

主要成果:

斯芬戈辛-1-酸盐 (S1P) 代谢酶和受体在妊娠组织中表达的差异.
宫内膜的S1P受体表达和S1P丰度高于分娩时的决定性和胆子膜.
与没有分娩相比,S1P和SPHK1表达在分娩时的肌子宫显著增加.

结论:

斯芬哥辛-1-酸盐 (S1P) 的新陈代谢和信号在人类妊娠组织中受到明显的调节.
研究结果表明,S1P在控制肌肉膜收缩性方面可能具有治疗作用.
组织特异性S1P调控提供了对产科并发症机制的洞察.

更多相关视频

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

Published on: January 26, 2024

Isolation of Leukocytes from the Human Maternal-fetal Interface

Isolation of Leukocytes from the Human Maternal-fetal Interface

Published on: May 21, 2015

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...