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单细胞成像揭示了在发育生物膜中自发的表型分类和投注对冲

Jung-Shen Benny Tai, Kee-Myoung Nam, Changhao Li

bioRxiv : the preprint server for biology

|June 12, 2025

概括

在Vibrio cholerae生物膜中的表型异质性创造了空间模式. 高循环二GMP (c-di-GMP) 细胞形成核心,而低c-di-GMP细胞形成外围,使其能够适应不断变化的环境.

科学领域:

微生物学微生物学
系统生物学系统生物学
生物物理学的生物物理.

背景情况:

现型异质性在生物膜发育中的作用尚不清楚.
循环二GMP (c-di-GMP) 对于细菌从运动状态过渡到静止状态至关重要.

研究的目的:

研究表型异质性如何影响Vibrio cholerae中的生物膜架构和发展.
在生物膜形成过程中以单细胞分辨率可视化和量化细胞内c-di-GMP水平.

主要方法:

开发用于跟踪细胞内c-di-GMP水平的先进成像工具.
发育中的Vibrio cholerae生物膜的单细胞分辨率分析.
整合单个血统追踪,突变分析和基于代理的建模.

主要成果:

在生物膜中确定了c-di-GMP水平的双模分布.
观察到的空间分类:高c-di-GMP细胞在核心,低c-di-GMP细胞在外围.
证明差异粘度和表面摩擦通过矩阵依赖相互作用驱动这种模式形成.

结论:

现型异质性和空间分类促进了浮游生物细胞的持续出现和脱落,增强了细菌的适应性.
在多细胞系统中引入了"差分阻力机制"用于模式形成.
通过强调表型异质性的功能重要性,扩大对生物膜生命周期的理解.

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