白色和棕色脂肪组织共享一个共同的纤维脂肪原始人群
bioRxiv : the preprint server for biology
|June 12, 2025
概括
这项研究确定了两种关键细胞类型,即白色和棕色脂肪组织模型中的承诺脂原体前体 (CAP) 和纤维脂原体前体样细胞 (FAPL). 这些发现验证了用于研究脂肪组织异质性的体外模型.
科学领域:
- 细胞生物学 细胞生物学
- 脂肪组织生物学 脂肪组织生物学
- 单细胞基因组学 单细胞基因组学
背景情况:
- 脂肪组织的异质性影响其在健康和疾病中的功能.
- 现有的体外模型在研究这种多样性方面存在局限性.
- 了解原始细胞种群对于脂肪组织研究至关重要.
研究的目的:
- 为了研究体外条件对白色和棕色前脂质细胞原始体身份的影响.
- 在脂肪组织中识别和表征不同的原始子群.
- 为了验证脂肪组织异质性的体外模型的生理相关性.
主要方法:
- 培养初级白色和棕色预脂细胞的单细胞RNA测序.
- 轨迹和调节分析以推断细胞状态过渡.
- 在体外和体内单细胞数据集的整合.
主要成果:
- 确定了两个主要的亚群:承诺的脂原体前体 (CAPs) 和纤维脂原体样细胞 (FAPLs).
- 在白色和棕色脂肪组织中发现的FAPLs,表现出保存的亲纤维和应激反应特征.
- CAP显示出仓库特定的身份,而FAPL则聚集在一起,表明保存的特征.
- 试验室种群与先前在体内确定的脂肪寄存的原始基因亚型相对应.
结论:
- 该研究验证了用于研究脂肪组织异质性和发育的体外模型.
- 纤维基原体样细胞代表了在仓库中保存的原体亚型.
- 参与脂原体前体表现出仓库特定的特征.
更多相关视频
10:28Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Published on: July 17, 2018
11.8K
06:08Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
2.1K
相关概念视频
01:26Loose Connective Tissue
7.1K
Loose connective tissue is found between many organs. Its main function is to absorb shock and bind tissues together. It also allows water, salts, and various nutrients to diffuse into cells that are embedded in it or present in adjacent tissues.
Adipose Tissue
Adipose tissue consists primarily of fat storage cells called adipocytes and little extracellular matrix. A large number of capillaries present within adipose tissue allow rapid mobilization of lipid molecules. White adipose tissue is...
Adipose Tissue
Adipose tissue consists primarily of fat storage cells called adipocytes and little extracellular matrix. A large number of capillaries present within adipose tissue allow rapid mobilization of lipid molecules. White adipose tissue is...
7.1K
01:02Hypodermis
4.2K
The hypodermis (the subcutaneous layer or superficial fascia) is present directly below the dermis. It connects the skin to the underlying fascia (fibrous tissue) of the bones and muscles. It is not strictly a part of the skin, although the border between the hypodermis and dermis can be difficult to distinguish. The hypodermis consists of well-vascularized, loose, areolar connective tissue and adipose tissue, which functions as a mode of fat storage and provides insulation and cushioning for...
4.2K