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Updated: Jun 13, 2025

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
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开发一种耐充电的嵌入式介质,用于对细胞和组织的高性能串行块面部成像

Thomas Deerinck, Steven Peltier, Mark Ellisman

    bioRxiv : the preprint server for biology
    |June 12, 2025
    PubMed
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    这项研究引入了一种新型聚乙烯糖醇 (PEG 3350) 合环氧树脂,可显著降低连续块面扫描电子显微镜 (SBEM) 期间的样本充电量. 这种方法可以提高生物样本的高分辨率3D成像,而不会影响图像质量.

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    Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
    The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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