相关实验视频

Updated: Jun 13, 2025

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

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与功能相关的膜蛋白动力学:整合生物信息学,分子动力学和单分子FRET

Hugh R Higinbotham, Christine A Arbour, Barbara Imperiali

    bioRxiv : the preprint server for biology
    |June 12, 2025
    PubMed
    概括

    我们开发了一种结合结构生物信息学,分子模拟和单分子FRET显微镜的新方法,观察完整膜蛋白在与连接体结合时如何改变形状,帮助药物发现.

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    Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules10:23

    Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

    Published on: April 25, 2025

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    Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET10:59

    Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

    Published on: August 17, 2022

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    相关概念视频

    Protein Dynamics in Living Cells01:19

    Protein Dynamics in Living Cells

    Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
    Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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    Protein Diffusion in the Membrane01:24

    Protein Diffusion in the Membrane

    Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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    Membrane Fluidity01:26

    Membrane Fluidity

    Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
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    Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
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