相关实验视频
Updated: Jun 13, 2025

13:00
Studying Wnt Signaling During Patterning of Conducting Airways
Published on: October 16, 2016
7.3K
在呼吸系统发育过程中,细胞命运的规范需要含有ARID1A的正规BAF复杂活性
bioRxiv : the preprint server for biology
|June 12, 2025
概括
对于肺部发育至关重要的BAF复合体,对于适当的气道和气泡细胞形成至关重要. 它的缺失,特别是ARID1A,破坏了肺部的模式和细胞分化,影响了肺部的器官生成.
科学领域:
- 发育生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 哺乳动物的肺部发育涉及复杂的过程,如分支形态发生和细胞谱系规范.
- 已知转录和信号调节器,但表观遗传控制仍然不太了解.
- 作为一种依赖ATP的染色质重塑剂的BAF复合体,正在研究其在肺上皮细胞发育中的作用.
研究的目的:
- 调查法定BAF复合体在哺乳动物肺上皮细胞发育中的作用.
- 确定BAF复合体及其亚单元ARID1A在肺形成和模式中的特定功能.
- 探索BAF复杂功能障碍对细胞命运获取和信号通路的影响.
主要方法:
- 在发育中的肺部中删除BAF复合体活动或ARID1A的基因操纵.
- 分析肺部形态,细胞类型规范和基因表达模式.
- 使用胚胎肺器官来测试分化缺陷的救援策略.
主要成果:
- 在全球BAF复合体被删除后,肺形成的完全丧失.
- 删除ARID1A导致近距离轴规范和AT1细胞分化失败.
- 功能障碍的BAF复合体导致了YAP/WNT激活,BMP信号损失和表皮分化受损.
- BMP4信号可以拯救AT1/AT2差异化,但WNT/YAP信号需要功能BAF.
结论:
- BAF复合体对于肺形成,近端-远端模式和细胞命运获取至关重要.
- ARID1A对于确定肺轴和膜细胞特异性至关重要.
- 在肺部发育过程中,BAF复杂活性差异调节信号通路 (BMP,WNT,YAP).
更多相关视频
08:04Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick
Published on: November 17, 2016
9.4K
10:01An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
Published on: September 19, 2018
9.0K
相关概念视频
Determination
18.2K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
18.2K
Non-Canonical Wnt Signaling Pathways
7.2K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.2K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Cis-regulatory Sequences
9.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.8K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Canonical Wnt Signaling Pathway
8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K