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相关概念视频

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
927
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

735
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.2K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

4.7K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
4.7K
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

579
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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相关实验视频

Updated: Jun 13, 2025

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
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Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

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通过使用时间编码样本多重复合来增加质谱的吞吐量.

Jason Derks, Kevin McDonnell, Nathan Wamsley

    bioRxiv : the preprint server for biology
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    此摘要是机器生成的。

    研究人员开发了timePlex,这是一种提高液体染色体质谱 (LC-MS) 蛋白质组学样本分析速度的新方法. 通过分级样本分离,timePlex可以组合地缩放吞吐量,从而使单个细胞的敏感分析成为可能,并显著提高了蛋白质组学效率.

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    相关实验视频

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    科学领域:

    • 蛋白质组学是指蛋白质组学.
    • 分析化学 分析化学
    • 生物技术是生物技术.

    背景情况:

    • 液态染色体质谱法 (LC-MS) 为分析物量化提供了高灵敏度,但面临着吞吐量限制.
    • 目前的多重复合策略,如plexDIA,通过在质域中进行多重复合来线性增加吞吐量.
    • 存在一种方法的需要,这种方法可以将蛋白质组学吞吐量相结合地扩大到线性增长之外.

    研究的目的:

    • 开发一种新的时间域多重复合策略,称为timePlex,以补充现有的质域多重复合.
    • 在基于LC-MS的蛋白质组学中实现样本吞吐量的组合缩放.
    • 为了证明timePlex对敏感,高通量蛋白质组分析的有效性.

    主要方法:

    • 开发了timePlex,这是一种在时间域中分离样本周期的分离和重叠方法.
    • 结合时间Plex与plexDIA (质域多重复合) 进行正交,组合多重复合.
    • 使用3-timePlex和3-plexDIA证明了9个样本的多重复合,使用3-timePlex和9-plexDIA证明了27个样本的多重复合.

    主要成果:

    • 使用27倍复数 (3倍复数和9倍复数DIA) 实现了每天超过500个样本的吞吐量.
    • 证明timePlex支持敏感分析,包括单细胞蛋白质组学.
    • 展示了通过结合时间域和质域多重复合来增加吞吐量.

    结论:

    • timePlex是LC-MS蛋白质组学有效的无标签复杂化方法.
    • 时间Plex和plexDIA的组合使得蛋白质组学吞吐量的组合扩展成为可能.
    • 预计未来的吞吐量增加超过每天1000个样本与这种综合方法.