相关实验视频

Updated: Jun 13, 2025

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.5K

通过使用时间编码样本多重复合来增加质谱的吞吐量

Jason Derks, Kevin McDonnell, Nathan Wamsley

    bioRxiv : the preprint server for biology
    |June 12, 2025
    PubMed
    概括

    研究人员开发了timePlex,这是一种提高液体染色体质谱 (LC-MS) 蛋白质组学样本分析速度的新方法. 通过分级样本分离,timePlex可以组合地缩放吞吐量,从而使单个细胞的敏感分析成为可能,并显著提高了蛋白质组学效率.

    更多相关视频

    Analyzing Large Protein Complexes by Structural Mass Spectrometry15:35

    Analyzing Large Protein Complexes by Structural Mass Spectrometry

    Published on: June 19, 2010

    24.1K
    Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization12:11

    Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

    Published on: February 27, 2020

    6.8K

    相关概念视频

    Tandem Mass Spectrometry01:21

    Tandem Mass Spectrometry

    Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
    Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
    927
    Mass Spectrometry: Complex Analysis01:21

    Mass Spectrometry: Complex Analysis

    Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
    GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
    735
    Peptide Identification Using Tandem Mass Spectrometry01:33

    Peptide Identification Using Tandem Mass Spectrometry

    Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
    This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
    6.4K
    Mass Spectrometers01:16

    Mass Spectrometers

    This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
    5.2K
    Mass Spectrometry: Overview01:19

    Mass Spectrometry: Overview

    Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
    4.7K
    Mass Analyzers: Common Types01:19

    Mass Analyzers: Common Types

    The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
    579