框 C/D snoRNP 和 MDT-15/MED15 通过脂肪酸代谢调节线粒体监测
bioRxiv : the preprint server for biology
|June 12, 2025
概括
线粒体监测途径对于多细胞生物的健康至关重要. 这项研究表明,MDT-15和脂肪酸脱酶FAT-6/FAT-7对于激活乙醇应激和反应元素 (ESRE) 途径至关重要,增强了对Pseudomonas aeruginosa的防御能力.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 多细胞生物拥有防御网络,以维持对压力因素的平衡.
- 线粒体功能障碍与各种病理有关,需要了解监控途径.
- 乙醇应激和反应元素 (ESRE) 途径被各种压力激活,包括细菌病原体.
研究的目的:
- 研究ESRE激活背后的分子机制.
- 确定参与对宿主防御Pseudomonas aeruginosa的关键基因和途径.
- 阐明脂肪酸代谢和盒子C/DsnoRNP在线粒体监测中的作用.
主要方法:
- RNA干扰 (RNAi) 破坏了C. elegans中的基因功能.
- 在各种压力条件下分析ESRE激活.
- 脂肪酸补充剂来拯救基因淘汰表型.
- 在细菌感染期间评估虫存活率.
主要成果:
- 为了激活ESRE,需要MDT-15及其下游效应器FAT-6和FAT-7.
- 补充特定脂肪酸可以挽救ESRE激活在敲击突变.
- 在P. aeruginosa感染期间破坏mdt-15或fat-6受损的虫生存.
- 盒子C/DsnoRNP复合体是必要的,以调节脂肪酸代谢在压力下.
结论:
- MDT-15和脂肪酸脱酶FAT-6/FAT-7在ESRE通路和天生的免疫力中发挥着关键作用.
- 脂肪酸代谢是细胞应激反应和宿主防御的关键组成部分.
- 盒子C/DsnoRNP,脂质,MDT-15/Mediator和线粒体监测之间存在一种新的相互作用.
更多相关视频
06:53Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase COX/SDH Double-labeling Histochemistry
Published on: November 23, 2011
36.7K
11:53Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
Published on: September 18, 2014
15.5K
相关概念视频
Translocation of Proteins into the Mitochondria
3.1K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.1K
Mitochondrial Protein Sorting
4.3K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death. Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.3K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Mitochondrial Membranes
9.2K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
9.2K
Porin Insertion in the Outer Mitochondrial Membrane
2.9K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
2.9K
Mitochondrial Precursor Proteins
2.5K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70 chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
Most of the mitochondrial...
2.5K
