相关实验视频
Updated: Jun 13, 2025

09:00
Cargo Loading onto Kinesin Powered Molecular Shuttles
Published on: November 3, 2010
10.5K
合成货物适配器揭示了可以增强dynein激活的分子特征
bioRxiv : the preprint server for biology
|June 12, 2025
概括
细胞质的dynein-1 (dynein) 运动活动是由适应蛋白调节的. 这些适配器的灵活性增加增强了运动复合体的运动性和效率.
科学领域:
- 细胞生物学 细胞生物学
- 分子电机分子电机
- 蛋白质的结构-功能
背景情况:
- 细胞质的dynein-1是真核生物中主要的逆行微管子电机.
- 主动运输需要dynein来结合dynactin和货物适配器,形成一个主动运输复合体.
- 现有的适配器具有结构上的相似性,但产生具有不同运动性的复合体.
研究的目的:
- 研究适配器的分子特征,这些特征决定了活跃运输复合体活动.
- 确定适配器序列变异如何影响dynein-dynactin复合体运动.
主要方法:
- 通过修改内源适配器 (NINL,BicD2,KASH5,Hook3) 来生成合成适配器.
- 使用了体外结合和基于TIRF的运动性试验.
- 在高活性合成适配器上进行结构功能分析.
主要成果:
- 适配器卷-卷域是必要的,并且足以激活dynein.
- 内生适配器可以修改为增加运动性,这表明没有进化压力最大速度.
- 一个过度活跃的合成适配器显著提高了速度,运行长度和频率.
- 在适配器中增加随机线圈与更高的运动性相关.
结论:
- 适配器的灵活性,特别是随机的线圈区域,对于调节dynein-dynactin复合体运动性至关重要.
- 增加的灵活性可能有助于动力校对来破坏不活跃复合物的稳定.
- 适配器序列的变化提供了对dynein传输的差异调节机制.
相关概念视频
Microtubule Associated Motor Proteins
7.7K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
7.7K
The Movement of Organelles and Vesicles
4.4K
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.4K
Adaptability of Cytoskeletal Filaments
3.7K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
3.7K
Mechanism of Ciliary Motion
3.6K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
3.6K
Cytoskeletal Coordination in Cell Migration
4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Microtubules in Cell Motility
3.2K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
3.2K

