相关实验视频

Updated: Jun 28, 2026

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
07:58

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Published on: June 19, 2013

13.0K

一个优化的活/死测试使用流细胞计量量化后压力和抗真菌治疗生存在各种酵母的酵母

Hanxi Tang, Jinye Liang, Bin Z He

    bioRxiv : the preprint server for biology
    |June 12, 2025
    PubMed
    概括

    这项研究提出了一种快速,可扩展的流细胞计测试方法,使用SYTO 9/Propidium Iodide染料来量化酵母在压力后的存活率. 它可以识别中间受损的细胞,为传统的殖民地形成单元 (CFU) 方法提供更快的替代方案.

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    Temporal Quantification of MAPK Induced Expression in Single Yeast Cells07:59

    Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

    Published on: October 4, 2013

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    High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity09:09

    High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

    Published on: November 16, 2016

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    相关概念视频

    Microbial Growth Measurement: Direct Methods01:23

    Microbial Growth Measurement: Direct Methods

    Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
    Methods to Assess Microbial Populations01:30

    Methods to Assess Microbial Populations

    Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
    Microbes in Food Production01:29

    Microbes in Food Production

    Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
    Bioreactor Controls-I01:28

    Bioreactor Controls-I

    Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
    Bioreactor Controls-III01:22

    Bioreactor Controls-III

    Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
    Methods of Medium Optimization01:28

    Methods of Medium Optimization

    Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...