Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

11.8K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
11.8K
Replicative Cell Senescence02:15

Replicative Cell Senescence

3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
Mutations01:39

Mutations

81.0K
Overview
81.0K
Aging01:26

Aging

39
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
39

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same author

Navigating a crowded developing brain leaves neurons with broken DNA.

Nature·2026
Same author

Systemic Lupus Erythematosus in Complications of Mastectomies With or Without Reconstruction.

The Journal of surgical research·2026
Same author

Duplex-Indel: a Snakemake pipeline for somatic Indel calling in Tn5 transposase-based duplex sequencing data.

Bioinformatics (Oxford, England)·2026
Same author

Somatic cancer variants enriched in Alzheimer's disease microglia-like cells drive inflammatory and proliferative states.

Cell·2026
Same author

Genomic approaches for understanding the evolution of the human brain.

Nature neuroscience·2026

相关实验视频

Updated: Jun 13, 2025

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
10:13

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Published on: August 12, 2014

13.4K

细胞类型特定的模式和体突变在发育和老化大脑的后果.

Andrea J Kriz, Shulin Mao, Diane D Shao

    bioRxiv : the preprint server for biology
    |June 12, 2025
    PubMed
    概括

    双重多体细胞精确地识别单个细胞中的体质突变,揭示细胞类型特定的衰老模式和脑组织中的基因表达变化. 这种方法促进了对体质马赛克及其对健康和疾病的影响的理解.

    更多相关视频

    Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
    09:51

    Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

    Published on: May 7, 2014

    15.3K
    Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
    09:25

    Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

    Published on: May 8, 2020

    10.6K

    相关实验视频

    Last Updated: Jun 13, 2025

    Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
    10:13

    Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

    Published on: August 12, 2014

    13.4K
    Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
    09:51

    Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

    Published on: May 7, 2014

    15.3K
    Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
    09:25

    Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

    Published on: May 8, 2020

    10.6K

    科学领域:

    • 基因组学和分子生物学
    • 神经科学是一个神经科学.
    • 癌症研究 癌症研究

    背景情况:

    • 准确地描述跨细胞类型的体质马赛克主义对于理解癌症,衰老和健康组织功能至关重要.
    • 现有的低通量技术限制了对细胞类型特定的体质突变及其功能影响的研究.
    • 开发高通量方法对于检测体质突变及其表型后果至关重要.

    研究的目的:

    • 开发一种高通量方法,即双重多组,用于从单个核中同时识别体质单核酸变体 (sSNV) 和多原子概况 (snATAC-seq,snRNA-seq).
    • 准确量化罕见细胞群中的sSNVs并描述它们的突变光谱.
    • 研究死后脑组织中细胞类型特定的体质突变率和模式及其与基因表达和疾病状态的相关性.

    主要方法:

    • 双重共识测序与单核多原子测试 (snATAC-seq,snRNA-seq) 集成,以提高准确性和减少测序错误.
    • 在snATAC-seq库构建中引入了字符串标记,以消除人工突变签名.
    • 双重多元组方法使用混合细胞系进行了验证,并应用于51,400多个从死后人类大脑组织中获取的细胞核.

    主要成果:

    • 双重多元体在低频细胞群中识别sSNV的高精度 (>92%) (2%) 并准确捕获已知的突变光谱.
    • 该方法揭示了大脑中不同的神经元和质细胞类型的与年龄相关的突变率和模式.
    • 克隆性sSNV在老年大脑的细胞质中被发现,并且与神经类型和自闭症谱系障碍 (ASD) 个体的基因表达改变相关.

    结论:

    • 双重多元体显著提高了在多种细胞类型和奥米克层中体性突变检测的准确性和吞吐量.
    • 这些发现表明,不同的脑细胞类型表现出独特的与年龄相关的体质突变特征,为大脑衰老提供了新的见解.
    • 身体突变直接影响基因表达表现型,涉及到正常衰老和神经发育障碍,如ASD.