相关实验视频
Updated: May 8, 2026

11:39
High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
13.3K
从纤维细胞生物质中构建生物糖化过程,使用纤维状真菌Trichoderma reesei
Masakazu Ike1,2, Kenji Yamagishi2, Ken Tokuyasu2
11 Research Center for Agricultural Robotics, NARO.
Journal of applied glycoscience
|June 12, 2025
概括
这项研究开发了一种高效的生物糖化工艺,使用Trichoderma reesei (T. reesei) 菌来生产糖. 在优化条件下,在120小时内从米和纤维素中获得高糖产量.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 微生物学 微生物学
背景情况:
- 细胞酶的生产和酶的糖化对于生物质的转化至关重要.
- 特里科德尔玛 (Trichoderma reesei) 是一个成熟且高效的细胞酶生产者.
- 将酶生产与糖化整合起来,可以简化生物过程.
研究的目的:
- 开发一种使用Trichoderma reesei的综合生物糖化工艺.
- 优化关键因素,以提高从基纤维素生物质中获得的糖产量.
- 为了比较这种有氧真菌过程的效率与无氧方法.
主要方法:
- 利用一种有氧真菌,Trichoderma reesei M2-1菌株,同时生产酶和糖化.
- 采用了两阶段的工艺:在28°C的真菌生长和在50°C的糖化.
- 优化的参数包括菌体注射剂大小和生长阶段持续时间.
主要成果:
- 120小时后,从处理的米中获得了74.5%的糖产量,从微晶纤维素中获得了60.6%.
- 与无氧过程相比,在较短的时间内证明了更高的糖产量.
- 鉴定了由原料特性影响的糖产量的显著变化.
结论:
- 使用Trichoderma reesei开发的生物糖化工艺对于生物质糖生产是有效的.
- 优化注射剂大小和生长周期显著影响到糖产量.
- 非转基因真菌菌株为生物质糖化提供了无氧微生物过程的有利替代品.
相关概念视频
Microbial Fermentation
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

