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Updated: Jun 17, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

在急性胆囊炎中寻找细菌,使用下一代测序器

Tomohiro Otsuka¹, Yoichi Ishizaki², Jiro Yoshimoto²

¹Juntendo University Institute for Environment and Gender-Specific Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.

Surgery open science

|June 12, 2025

概括

胆囊不是无菌的;急性胆囊炎涉及不同的细菌菌群. 在健康的胆囊中没有发现的特定细菌可能会导致疾病的发展.

科学领域:

胃肠病学胃肠病学
微生物学微生物学
手术病理学手术病理学

背景情况:

胆囊传统上被认为是无菌的.
最近的研究使用16S核糖体RNA (16SrRNA) 基因分析揭示了健康胆囊中的胆道微生物组.
这项研究研究了急性胆囊炎的胆道微生物组.

研究的目的:

从急性胆囊炎患者的胆汁样本中识别细菌菌群.
为了将细菌组成与正常胆囊的成分进行比较.
探索特定细菌在急性胆囊炎的发病过程中的潜在作用.

主要方法:

从一级或二级急性胆囊炎的患者收集胆汁样本,这些患者接受了腹腔镜胆囊切除术.
使用标准细菌学 (培养试验) 和16S rRNA基因测序证实了细菌的存在.
样品在诊断后24小时内进行分析.

主要成果:

在所有样本中通过16S rRNA基因测序检测出细菌胆道菌群,包括通过培养的负面样本.
在29个样本中,15个是培养阳性的.
除了在正常胆囊中发现的其他细菌 (例如,Propionibacterium*,Coprococcus*,Prevotella*) 在86%的病例中被发现.

结论:

胆囊含有细菌菌群,即使在急性胆囊炎中也是如此.
特定的细菌,如Propionibacterium,Coprococcus,Prevotella,Sediminibacterium和Collinesella,与急性胆囊炎有关.
这些细菌可能在急性胆囊炎的发展中发挥作用.

关键词:

16S rRNA 是一个16S rRNA.16S 核糖体RNA 的 16S 核糖体RNA.急性胆囊炎是一种疾病.细菌的植物群.胆道微生物组胆道微生物组微生物组是一个微生物组.这是下一代测序器.

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