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相关概念视频

Role of Septins01:02

Role of Septins

1.7K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.7K
Septins01:19

Septins

1.8K
Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
1.8K
Microtubules in Signaling01:22

Microtubules in Signaling

1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

2.0K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.0K

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相关实验视频

Updated: Jun 14, 2025

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
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Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

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博格3控制 septin 聚合,以形成初级乳毛.

Janik N Schampera1,2, Friederike Lehmann1,3,4, Ana Valeria Meléndez2,3,5

  • 1Institute for Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.

iScience
|June 12, 2025
PubMed
概括

七素以八合体的形式进入初级乳头,并为丰富而聚合. 博格3 (Cdc42ep5) 对于隔膜线索的定位和内动态至关重要,由底部的Cdc42活性调节.

关键词:
生物化学 生物化学细胞生物学 细胞生物学细胞生物学的组织方面.

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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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相关实验视频

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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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科学领域:

  • 细胞生物学 细胞生物学
  • 细胞骨动力学 细胞骨动力学
  • 乳生物学的生物学

背景情况:

  • 七GTPases是细胞骨蛋白质,形成聚合物,对细胞功能至关重要.
  • 众所周知,七会局部化到主要的乳毛,充当信号中心.
  • 它们在狭窄的眼环境中的特定组合和调节仍然不清楚.

研究的目的:

  • 为了研究小分子状态,聚合,和调节的 septins 在初级毛内.
  • 为了确定控制隔膜线丝动态和乳毛中的丰富的因素.
  • 阐明Borg3 (Cdc42ep5) 和Cdc42在隔膜纤维局部中的作用.

主要方法:

  • 利用培养细胞研究初级乳毛中隔膜的行为.
  • 采用Borg3的淘汰策略和操纵Cdc42活动.
  • 观察到隔膜局部化,寡合化和聚合化动态.

主要成果:

  • 发现七素以八合体的形式进入乳毛细胞,并且需要聚合来进行丰富.
  • 确定Borg3 (Cdc42ep5) 是状隔膜线 filaments定位的重要组成部分.
  • 博格3淘汰赛或Cdc42失调损害了隔膜动力学和状细胞丰富.
  • 博格3定位受控制于底的Rho-GTPase Cdc42活动周期.

结论:

  • 七八合体在乳毛内聚合,这一过程对其丰富至关重要.
  • 博格3是隔膜丝组装和局部化的关键调节者,在初级乳毛中.
  • 毛底部的Cdc42活动决定了Borg3的定位,因此,毛中的隔膜动态.