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Updated: Jun 14, 2025

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

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基于qPCR的基因量化中的差异及其依赖于土壤特性,抑制剂的存在和DNA提取套件类型

Eunhye Lee1, Hyun Jeong Lim1, Ahjeong Son1

  • 1Department of Environmental Science and Engineering, Ewha Womans University 52 Ewhayeodae-gil, Seodaemun-gu Seoul 03760 Republic of Korea hj.lim77@ewha.ac.kr ason@ewha.ac.kr ahjeong.son@gmail.com +82-2-3277-3773 +82-2-3277-3339.

RSC advances
|June 12, 2025
PubMed
概括

土壤DNA提取套件的选择显著影响定量PCR (qPCR) 中的基因定量精度. 复杂土壤中的DNA质量差和抑制剂加剧了这些差异,突出了需要仔细选择方法的需要.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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