预测基液质量的新策略:可视检测功能细菌及其应用
Lijuan Yin1, Lijing Sun1, Yueqi Wang1
1State Key Laboratory of Bio-based Fiber Materials, State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Current research in food science
|June 12, 2025
概括
一个新的CRISPR/Cas12a生物传感器准确量化了Acetilactobacillus jinshanensis,这是Baijiu生产中的一个关键微生物. 更高的度与更优质的基液质量相关,使发酵能够得到更好的控制.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 白发酵涉及多种微生物,其中Acetilactobacillus jinshanensis对于江口味的白品质至关重要.
- 精确量化A. jinshanensis对于控制发酵和确保基液质量至关重要.
研究的目的:
- 开发一种新型的CRISPR/Cas12a合金纳米粒子视觉生物传感器,用于量化A. jinshanensis.
- 将传感器的性能与现有方法进行评估,并将其应用于真实的Baijiu发酵.
主要方法:
- 使用金纳米粒子开发基于CRISPR/Cas12a的视觉生物传感器.
- 对A. jinshanensis的定量检测,检测极限为1副本/μL.
- 生物传感器的应用用于监测不同Baijiu发酵车间的A. jinshanensis度.
主要成果:
- 开发的生物传感器表现出高灵敏度,超过定量实时PCR.
- 在A. jinshanensis度和基液质量之间发现了显著的线性关系 (R2 = 0.845).
- 在优秀级 (4838) 和普通 (2145) 车间观察到明显的A. jinshanensis度.
结论:
- 克里斯普尔/Cas12a生物传感器提供了一种灵敏而准确的方法来定量A. jinshanensis.
- A. jinshanensis的度可以作为基液质量的预测标记.
- 这种方法促进了可控制的发酵过程,并提高了Baijiu的质量.
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