细胞散毒素增加的DNA损伤和涉到YAP/TAZ-TEAD信号通路
Ruxue Jia1, Lamia Azzi-Martin1,2, Mariana Saraiva1
1Univ. Bordeaux, INSERM, BRIC, U 1312, F-33000 Bordeaux, France.
The Journal of infectious diseases
|June 12, 2025
概括
细菌基因毒素,如CDT和 colibactin,激活了YAP/TAZ-TEAD通路,该通路控制了DNA损伤后的细胞. 这一途径对于表皮细胞响应基因毒性细菌感染至关重要.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 细菌基因毒素,包括CDT和 colibactin,在宿主细胞中诱导显著的DNA损伤和基因组不稳定.
- 这些基因毒素破坏了DNA损伤反应途径.
- 观察到的细胞表型表明基因毒素效应与河马信号通路之间的潜在联系.
研究的目的:
- 为了研究海马信号通路在上皮细胞中毒后CDT/CdtB和大肠杆菌素中的作用.
- 阐明细菌基因毒素影响宿主细胞信号传递和基因组完整性的分子机制.
主要方法:
- 使用了正常和癌症衍生的上皮肠和肝细胞系.
- 暴露于CDT/CdtB和大肠杆菌素的细胞.
- 分析了Hippo路径组件,包括YAP/TAZ和TEAD介导的转录.
- 抑制YAP/TAZ-TEAD结合对DNA损伤,修复和性的评估影响.
主要成果:
- CDT的活性CdtB子单元调节YAP/TAZ的表达,并增加TEAD介导的转录.
- 抑制YAP/TAZ-TEAD与脊素和K-975的结合减少了DNA损伤,损坏了修复,并降低了球性.
- 科利巴克丁暴露对YAP/TAZ-TEAD通路和细胞表型产生了类似的影响.
- YAP/TAZ-TEAD信号传递与CDT/CdtB诱导的DNA损伤中幸存的细胞中增加的性有关.
结论:
- 产生基因毒素的细菌感染涉及YAP/TAZ-TEAD信号通路.
- 这条通路在表皮细胞中DNA损伤后调节性中发挥着关键作用.
- 这些发现突出了一个新的机制,通过这种机制,细菌操纵宿主细胞过程,以促进基因组不稳定性.
关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.这种病原体是Helicobacter hepaticus.这是一个CDTCDT.河马河马是什么意思 海马河马是什么意思K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975 K-975我们的 TEAD TEAD 团队.在XMU-MP-1中使用XMU-MP-1.雅普/塔兹 (YAP/TAZ) 是一个国家.在 colibactin 的使用中.形形形形形形更多相关视频
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