在长期殖民期间,Enterococcus物种之间的异质性和代谢多样性
Philip A Karlsson1, Taoran Zhang1, Josef D Järhult2
1Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Microbiology spectrum
|June 12, 2025
概括
在导管患者中,抗微生物药物耐药的Enterococcus物种表现出不同的遗传和代谢特征. 了解这种多样性对于管理复杂的尿路感染和改善治疗结果至关重要.
科学领域:
- 医学微生物学 医学微生物学
- 传染性疾病 传染性疾病
- 遗传学 遗传学 是一个
背景情况:
- 尿路感染 (UTI) 越来越多地被抗菌素耐药性肠球菌种所复杂化,特别是在重症监护室 (ICU) 住院导管患者中.
- 传统上对单一血统的关注忽视了医院环境中Enterococcus种群中复杂的遗传和代谢多样性.
研究的目的:
- 在导管ICU患者中调查Enterococcus物种 (E. faecium,E. faecalis,E. durans) 的遗传和代谢多样性.
- 为了确定新的血统,序列类型 (ST) 和代谢适应,有助于抗生素耐药性和毒性.
- 评估横向基因转移 (HGT) 和等离子体变异在肠球菌感染中的作用.
主要方法:
- 在乌普萨拉大学医院检查了210名加导管ICU患者的尿样.
- 使用PhenePlateTM-RF类型识别39种独特的类型在E. faecium,E. faecalis和E. durans.中.
- 分析了遗传多样性,代谢适应 (例如,L-氨酸代谢),抗生素耐药性概况和等离子体含量.
主要成果:
- 在E. faecium中发现了显著的遗传和代谢多样性,特别是在克隆复合体17 (CC17) 中.
- 在个体患者中检测到多个序列类型 (ST) 和血统,包括不同ST的混合感染.
- 观察到代谢适应和抗生素耐药性的转变,有证据表明HGT和等离子体含量的变化.
- 虽然较少见,但E. durans与E. faecium共享代谢特征,并表现出多克隆特征;MALDI-TOF MS的错误识别被注意到.
结论:
- 导管患者的肠球菌感染复杂,具有显著的遗传和代谢多样性.
- 了解克隆多样性,代谢适应和HGT对于有效管理和治疗由Enterococcus物种引起的尿路感染至关重要.
- 准确的识别方法至关重要,正如E. durans.的错误识别所证明的那样.
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