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GSDMD和GSDME在肠道冠状病毒PDCoV诱导的热和炎症反应中表现出不同的作用

Chenyu Li^1,2, Yuting Shi^1,2, Chunying Xie^1,2

¹Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.

Journal of virology

|June 12, 2025

概括

猪三角型冠状病毒 (PDCoV) 触发了细胞灭绝,一种被编程的细胞死亡,GSDME在炎症中发挥了主导作用. PDCoV蛋白酶分裂GSDMD,阻碍其抗病毒功能并促进感染.

科学领域:

兽医病毒学兽医病毒学
免疫学免疫学免疫学
细胞病理学细胞病理学

背景情况:

猪三角冠状病毒 (PDCoV) 在猪中引起肠道疾病和炎症.
炎症性编程细胞死亡的 Pyroptosis 与病毒病原发生有关.
气体皮质D (GSDMD) 和气体皮质E (GSDME) 在PDCoV感染中的特定作用尚不清楚.

研究的目的:

为了调查PDCoV感染诱导热的原因.
阐明GSDMD和GSDME在PDCoV诱导的热和炎症中的不同作用.
确定PDCoV干扰热的机制.

主要方法:

猪皮上皮细胞系感染PDCoV.
分析PDCoV感染小猪感染的感染细胞和组织中的烧死标记物.
通过PDCoV非结构蛋白5 (3C类蛋白酶) 检测GSDMD和GSDME的裂变.

主要成果:

感染PDCoV会触发GSDMD和GSDME介导的灭.
GSDME在PDCoV诱导的热和随后的炎症反应中发挥着主导作用.
类似PDCoV 3C的蛋白酶可以切割GSDMD,但不能切割GSDME,从而降低了GSDMD的抗病毒和火性功能.

结论:

感染PDCoV诱导热,GSDME是炎症反应的主要媒介.
PDCoV利用其3C类蛋白酶来切割GSDMD,从而逃避宿主抗病毒防御.
了解GSDMD和GSDME的不同作用,可以深入了解PDCoV病原和潜在的治疗点.

关键词:

3C类蛋白酶 (3CLpro) 是一种3C类蛋白酶.气体皮层 D D D D D 气体皮层 E E E E 炎症反应是一种炎症反应.猪三角型冠状病毒 (PDCoV)

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