聚烯微塑料通过ROS介导的p53通路诱导人类角膜上皮细胞受伤
Jianfeng Long1, Limin Deng1, Jian Liu1
1Department of Clinical Nutrition, the 2nd Xiangya Hospital of Central South University, Changsha, 410011, China.
Mutagenesis
|June 12, 2025
概括
聚乙烯微塑料 (PS-MPs) 通过引起DNA损伤和细胞死亡来损害人类角膜细胞,这与氧化应激有关. 抗氧化剂可以减轻这些有毒效应,这表明抗微塑料诱导的细胞损伤的保护机制.
科学领域:
- 眼部毒理学 眼部毒理学
- 环境健康 环境健康
- 细胞生物学 细胞生物学
背景情况:
- 微塑料污染是一个日益严重的环境问题.
- 微塑料对眼睛组织的影响尚不清楚.
- 人类角膜上皮细胞 (HCEP) 是对眼睛外部因素的第一道防线.
研究的目的:
- 研究聚烯微塑料 (PS-MP) 对人类角膜上皮细胞 (HCEP) 的毒理影响.
- 阐明HPCEP中PS-MP诱导的细胞损伤的潜在机制.
主要方法:
- 使用CCK-8试验评估了细胞毒性.
- 用流式细胞计量分析了细胞周期,细胞亡和亡.
- 通过免疫光检测检测出反应性氧物种 (ROS) 和DNA损伤 (γ-H2AX).
- 与细胞死亡途径相关的蛋白质表达被用西式涂抹评估.
主要成果:
- PS-MP 细胞毒性取决于颗粒大小和度,较小的颗粒和较高的度会造成更大的伤害.
- 暴露于PS-MPs诱导HCEP细胞的细胞周期停止,亡和亡.
- PS-MPs导致过度的ROS产量和DNA损伤,这些都是通过ROS食尸剂减轻的.
- ROS清洁剂逆转了PS-MP诱导的关键蛋白质的变化,这些蛋白质参与细胞循环调节,亡和亡.
结论:
- 暴露于PS-MP会诱导HCEP中的显著细胞损伤,包括细胞循环停止,亡和亡.
- 这种损伤是由ROS过度产生和随后激活P53信号通路的介导.
- 这些发现突显了微塑料对眼睛的潜在毒性,并建议与ROS相关的途径作为治疗点.
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