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相关实验视频

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隔离和定量大型衰老中性粒细胞衍生的囊泡的协议.

Alan Y Hsu1, Qingxiang Huang2, Sizhou Feng2

  • 1Department of Pathology, PhD Program in Immunology, Harvard Medical School, Mass General Brigham, Dana-Farber/Harvard Cancer Center, Boston, MA 02115, USA.

STAR protocols
|June 12, 2025
PubMed
概括

这项研究详细介绍了从老鼠和人类样本中分离出大型老化中性粒细胞衍生的囊泡 (LAND-Vs) 的协议. 该方法使用离心和光激活细胞分类 (FACS) 进行净化.

关键词:
细胞生物学 细胞生物学细胞隔离细胞的隔离.流动细胞计量 (Flow Cytometry) 是一种流动细胞计.免疫学 免疫学 免疫学

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科学领域:

  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学
  • 细胞外囊泡 细胞外囊泡

背景情况:

  • 中性粒细胞是关键的免疫细胞,参与炎症和宿主防御.
  • 老化的中性粒细胞释放出大型老化的中性粒细胞衍生囊泡 (LAND-Vs),这些囊泡在生物过程中具有重要作用.
  • 了解LAND-Vs需要有效的隔离和净化方法.

研究的目的:

  • 详细介绍一项用于识别和净化小鼠大老化中性粒基衍生囊泡 (LAND-Vs) 的详细方案.
  • 描述人类LAND-Vs.的隔离和量化.
  • 为研究中性粒细胞外囊泡的研究人员提供可复制的方法.

主要方法:

  • 从体外中性粒细胞培养物,骨髓,血液和支气管支气管洗液 (BALF) 中分离小鼠LAND-Vs.
  • 使用微分离心和光激活细胞分类 (FACS) 净化LAND-Vs.
  • 从中性粒细胞和血液中分离和定量人类LAND-Vs的协议的调整.

主要成果:

  • 通过使用描述的协议,成功识别和净化小鼠LAND-Vs.
  • 来自各种ex vivo来源的LAND-Vs隔离的演示.
  • 人类LAND-Vs隔离和定量化的既定方法.

结论:

  • 提出的协议可以有效地隔离和净化小鼠和人类LAND-Vs.
  • 这种方法促进了对LAND-Vs.生物作用的进一步研究.
  • 该协议适用于体外和体外样本,从而提高了其实用性.