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Updated: Jun 16, 2025

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Published on: April 23, 2021

通过泛膨胀显微镜可视化核孔复杂的可塑性.

Kimberly J Morgan¹, Emma Carley¹, Alyssa N Coyne^2,3

¹Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.

The Journal of cell biology

|June 12, 2025

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查看所有相关文章

新的工具揭示了核孔综合体 (NPC) 的可塑性. 在疾病中,LINC复合体通过影响核外张力和POM121局部变化来影响NPC直径.

科学领域:

细胞生物学细胞生物学
生物物理学的生物物理.

背景情况:

调查核孔复合体 (NPC) 塑性,以应对细胞类型和环境至关重要.
需要可访问的工具来研究NPC大小和本地化动态.

研究的目的:

开发和利用用于可视化和量化NPC可塑性的新方法.
了解LINC复合体和核外张力在调节NPC直径中的作用.
检查NPC在疾病模型中的结构变化.

主要方法:

全扩展显微镜 (pan-ExM) 用于NPC的高分辨率成像.
基于机器学习的细分用于NPC识别和分析.
实验操纵包括高透性冲击和LINC复合体耗尽.

主要成果:

NPC 显示了广泛的直径,扩张偏向于基底核表面.
超透性冲击会导致NPC收缩,而LINC复合体枯竭会消除基底扩张偏差.
POM121在患有C9orf72肌缩侧面硬化症的患者衍生神经元中的NPC中重新定位.

结论:

泛扩展显微镜在研究单个NPC分辨率的NPC可塑性方面是有效的.

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Last Updated: Jun 16, 2025

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Published on: April 23, 2021

Single-Molecule Imaging of Nuclear Transport

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

A Direct Force Probe for Measuring Mechanical Integration Between the Nucleus and the Cytoskeleton

Published on: July 29, 2018

LINC复合体可以调节局部核外张力,以调节NPC扩张.

在疾病背景下可以观察到NPC结构变化,如POM121转移.