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通过泛膨胀显微镜可视化核孔复杂的可塑性
Kimberly J Morgan1, Emma Carley1, Alyssa N Coyne2,3
1Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
The Journal of cell biology
|June 12, 2025
概括
新的工具揭示了核孔综合体 (NPC) 的可塑性. 在疾病中,LINC复合体通过影响核外张力和POM121局部变化来影响NPC直径.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 调查核孔复合体 (NPC) 塑性,以应对细胞类型和环境至关重要.
- 需要可访问的工具来研究NPC大小和本地化动态.
研究的目的:
- 开发和利用用于可视化和量化NPC可塑性的新方法.
- 了解LINC复合体和核外张力在调节NPC直径中的作用.
- 检查NPC在疾病模型中的结构变化.
主要方法:
- 全扩展显微镜 (pan-ExM) 用于NPC的高分辨率成像.
- 基于机器学习的细分用于NPC识别和分析.
- 实验操纵包括高透性冲击和LINC复合体耗尽.
主要成果:
- NPC 显示了广泛的直径,扩张偏向于基底核表面.
- 超透性冲击会导致NPC收缩,而LINC复合体枯竭会消除基底扩张偏差.
- POM121在患有C9orf72肌缩侧面硬化症的患者衍生神经元中的NPC中重新定位.
结论:
- 泛扩展显微镜在研究单个NPC分辨率的NPC可塑性方面是有效的.
- LINC复合体可以调节局部核外张力,以调节NPC扩张.
- 在疾病背景下可以观察到NPC结构变化,如POM121转移.
相关概念视频
01:26Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
01:37Super-resolution Fluorescence Microscopy
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