油性酵母在试点工厂发酵器中的β-胡卜素生产:产量标准化和工艺扩大规模
Mirnalini Juyal1,2, Sudipto Adhikari2,3, Rahul Gautam1,2
1Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand 248005, India.
Journal of agricultural and food chemistry
|June 12, 2025
概括
这项研究表明,在大型发酵器中使用Rhodotorula mucilaginosa酵母进行可扩展的β-胡卜素生产. 具有成本效益的微生物色素工艺适合于工业集成.
科学领域:
- 生物技术是生物技术.
- 微生物颜料的生产方法
- 酵母发酵 酵母发酵的方法
背景情况:
- 油性酵母如Rhodotorula mucilaginosa是有价值的微生物颜料的来源.
- 从实验室扩大微生物颜料生产到工业水平,面临重大挑战.
- 对于生产β-胡卜素的高效和成本效益的方法有需求.
研究的目的:
- 使用Rhodotorula mucilaginosa建立一种可扩展的β-胡卜素生产过程.
- 优化发酵条件以最大限度地提高颜料产量.
- 为了证明试点规模的潜水发酵用于微生物颜料制造的可行性.
主要方法:
- 在发酵介质中优化碳和来源.
- 在试点规模 (500升) 发酵器中进行浸泡发酵,考虑介质的风力学和水力动力学.
- 使用溶剂提取和薄层染色学净化β-胡卜素.
- 使用紫外线可见光谱法,HPLC和FTIR光谱法分析颜料.
主要成果:
- 通过Rhodotorula mucilaginosa IIPL32 (MTCC 25056) 实现了选择性β-胡卜素的产生.
- 在一个500升的发酵器中发酵,每升20克的葡萄糖产生3.3公斤的酵母生物质和5.1克的β-胡卜素.
- 分析技术证实了贝塔胡卜素的主导存在.
结论:
- 开发的过程是可扩展的,用于工业级的微生物颜料生产.
- 该过程可以与现有的脂质生物炼油厂集成,资本支出最小.
- 这种方法为生产微生物脂质和颜料提供了一种具有成本效益的方法.
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