卵黄蛋白的序列酶化水解:水解剂的动力学,功能性和生物活性
Silvia N Rios-Morales1, Veymar G Tacias-Pascacio2, Maria Guadalupe Aguilar-Uscanga1
1Unidad de Investigación y Desarrollo en Alimentos, Tecnológico Nacional de México/ IT-Veracruz, Av. M. A. De Quevedo 2779, 91897 Veracruz, Ver., Mexico.
International journal of biological macromolecules
|June 12, 2025
概括
使用Alcalase和Flavourzyme的无莱西丁蛋黄 (LFEY) 的序列酶化水解显著提高了水解的蛋白质程度,可溶性蛋白质含量和生物活性特性,包括抗氧化和 ACE 抑制活性.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 酶学 是一种酶学.
背景情况:
- 没有莱西丁的蛋黄 (LFEY) 是一种富含蛋白质的副产品,具有价值化潜力.
- 酶性水解可以改善蛋白质的功能性和生物活性性质.
研究的目的:
- 通过使用Alcalase和Flavourzyme来研究LFEY序列酶化水解的有效性.
- 评估水解条件对LFEY水解剂分子量 (Mw) 形状,功能和生物活性的影响.
主要方法:
- 在不同的pH值和酶度下,使用酸盐 (A),芳香酶 (F) 或连续的A-F系统来液化LFEY.
- 化物被分析为Mw形状,可溶性蛋白,乳化活性指数 (EAI),乳液稳定性指数 (ESI),抗氧化剂和抗氧化剂,以及抗氧化酶转化酶抑制剂 (ACEI) 活动.
- 超过 (UF) 用于分离基于Mw (5和1kDa膜) 的水解剂.
主要成果:
- 酸酶有效地化LFEY蛋白,其活性取决于pH控制.
- 在序列反应中由Alcalase产生的Flavourzyme化.
- 与单酶处理相比,连续的A-F水解增加了水解的程度6%,可溶性蛋白质增加了9%.
- 通过序列水解,抗氧化剂和ACEI活性显著增强 (分别是近两倍和三倍).
- 具有Mw在1-5kDa之间的UF分量表现出优异的抗氧化剂和ACEI活动.
结论:
- 使用Alcalase和Flavourzyme的序列酶化水解是利用LFEY副产品的有效策略.
- A-F 序列系统展示了协同的酶合作,产生具有改进功能和生物活性特性的化物.
- 特定的分数 (1-5 kDa) 具有强大的抗氧化剂和ACE抑制潜力,可用于功能性食品或营养药品.
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