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鉴定和表达miRNA-mRNA调节模块的分析与卡梅利亚drupifera的脂质积累相关的表达

Xue Sun¹, Linqiang Ke¹, Yougen Wu¹

¹School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.

International journal of biological macromolecules

|June 12, 2025

概括

这项研究揭示了Camellia drupifera种子中脂质积累的复杂miRNA调节. 识别关键的miRNA-mRNA模块为改善这种重要的木质油作物提供了基础.

科学领域:

植物分子生物学植物分子生物学
农业科学农业科学
生物技术是生物技术.

背景情况:

卡梅莉亚drupifera是一个重要的木质油作物.
种子成熟需要大量的脂质积累.
微RNAs (miRNAs) 在种子发育和脂肪酸生物合成中起着调节作用.

研究的目的:

为了研究miRNA介导的调节网络,控制C. drupifera种子成熟期间的脂质积累.
确定关键的miRNA及其参与脂肪酸生物合成的基因.
为基因改进C. drupifera油含量提供分子基础.

主要方法:

在三个发育阶段对C. drupifera种子的转录组和小RNA测序.
对差异表达单基因 (DEGs) 和miRNAs的综合分析.
miRNA目标预测和功能丰富分析.

主要成果:

确定了22292个DEG,251个已知的miRNA和343个新的miRNA.
发现了407个可能由112个小RNA调节的DEGs,形成了一个复杂的调节网络.
确定了8个关键的miRNA-mRNA模块,与脂肪酸代谢,不和脂肪酸生物合成和糖脂代谢有关.

结论:

在C. drupifera种子发育过程中对miRNA调节网络的全面洞察.
通过基因改进建立了一个分子基础,通过基因改进增强该作物的油产量.

关键词:

卡梅莉亚果 (Camellia drupifera) 是一种植物.石油的积累石油的积累监管网络监管网络是指监管网络的组成部分.这是一个小RNARNA.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...