在卵巢癌的多个测试中编程死亡-连接体1表达:比较分析
Eun Bi Jang1, Kyeong A So1, Wook Youn Kim2
1Department of Obstetrics and Gynecology, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul, Korea.
Cancer research and treatment
|June 12, 2025
概括
在卵巢癌中评估编程细胞死亡配体1 (PD-L1) 表达,在测试之间显示出显著的变异性. 化疗可以增加PD-L1阳性,影响免疫检查点抑制剂 (ICI) 治疗决策.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 病理学 病理学 病理学
背景情况:
- 卵巢癌的治疗具有挑战性,对免疫检查点抑制剂 (ICI) 的反应有限.
- 编程细胞死亡连接体1 (PD-L1) 试验和切断值的标准化缺乏,使临床决策复杂化.
- PD-L1表达是ICI疗效的一个关键生物标志物.
研究的目的:
- 为了评估通常使用的PD-L1免疫组织化学分析在卵巢癌中的一致性.
- 评估化疗后PD-L1表达的变化.
- 在卵巢癌中为PD-L1提供标准化测试协议的信息.
主要方法:
- 分析了29个卵巢癌组织样本,使用了五个经过验证的PD-L1试验 (Dako 22C3,Ventana SP263,Ventana SP142,Dako 28-8,Ventana 22C3).
- 使用组合阳性得分 (CPS),免疫细胞 (IC) 或瘤比例得分 (TPS) 在1%,5%和10%的截止值评估PD-L1阳性.
- 协同分析 (OPA,科恩卡帕) 和化疗前与后的PD-L1表达评估.
主要成果:
- 在1%的CPS切线下,PD-L1阳性率从15.8% (SP142) 到29.8% (Dako 22C3,SP263) 不同.
- SP142测定显示出最低的一致性;Dako 22C3显示出与SP263,28-8和Ventana 22C3.3的高度一致性.
- 化疗增加了PD-L1阳性,28%的患者从阴性转化为阳性.
结论:
- 卵巢癌PD-L1表达是测试依赖的,突出了对标准化测试的需要.
- 化疗后增加PD-L1表达对于指导ICI治疗时间至关重要.
- 需要进一步的研究来验证发现,并完善PD-L1测试的临床应用.
相关概念视频
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:12Loss of Tumor Suppressor Gene Functions
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:12Loss of Tumor Suppressor Gene Functions
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
01:21Cancer Survival Analysis
Cancer survival analysis focuses on quantifying and interpreting the time from a key starting point, such as diagnosis or the initiation of treatment, to a specific endpoint, such as remission or death. This analysis provides critical insights into treatment effectiveness and factors that influence patient outcomes, helping to shape clinical decisions and guide prognostic evaluations. A cornerstone of oncology research, survival analysis tackles the challenges of skewed, non-normally...