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Updated: Jun 14, 2025

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使用微流体探头对生物组织进行高空间分辨率质谱成像

Li-Xue Jiang¹, Xiangtang Li¹, Matthias Polack²

¹James Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, IN, USA.

Nature protocols

|June 12, 2025

概括

我们开发了一种用户友好的微流体探针 (MFP) 用于纳米喷涂脱落电喷离电离化质谱成像 (纳米-DESI MSI). 这项创新提高了生物样本的高分辨率分子映射的吞吐量和可访问性.

科学领域:

分析化学分析化学
生物技术是生物技术.
质谱测量质量谱测量

背景情况:

纳米喷雾脱电喷雾电离 (纳米-DESI) 是一个强大的技术,用于生物样本的分子映射.
纳米-DESI质谱成像 (MSI) 的更广泛采用受到复杂性和吞吐量的限制.
高空间分辨率对于生物组织的详细分析至关重要.

研究的目的:

开发一个强大的和用户友好的微流体探头 (MFP) 纳米-DESI MSI.
为了提高纳米-DESI MSI的实验吞吐量和可访问性.
为纳米-DESI MSI提供全面的工作流,从探头制造到数据分析.

主要方法:

设计和制造一种新的微流体探针 (MFP).
将MFP集成到纳米-DESI MSI工作流中.
为高分辨率成像和吞吐量优化实验参数.

主要成果:

实现了8-10微米的高空间分辨率,用于分子映射.
在实验性吞吐量方面,证明了高达10倍的改善.
启用了具有细胞分辨率的大组织部分的成像.
开发了一个完整的工作流程,用于探头组装,数据采集和分析.

结论:

开发的MFP显著提高了纳米-DESI MSI的易用性和效率.
这一进步使得科学界更容易获得高分辨率的分子绘图.
易于使用的探针和工作流简化了生物样本的分析.

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