在摇动瓶培养过程中调整Aspergillus niger颗粒直径,种群异质性和核心架构
K Engelbert1, C Deffur2, T C Cairns3
1Institute of Biotechnology, Chair of Applied and Molecular Microbiology, Technische Universität Berlin, Straße des 17. Juni 135, 10623, Berlin, Germany. karin.engelbert@tu-berlin.de.
Biotechnology for biofuels and bioproducts
|June 12, 2025
概括
这项研究表明,通过调整子度,动频率和滑石粉,可以对Aspergillus niger潜水培养形态进行可重复控制. 优化条件产生更大的颗粒,增强蛋白质分泌和减少葡萄糖消耗.
科学领域:
- 生物技术是生物技术.
- 微生物生理学 微生物生理学
- 生物工艺工程 生物工艺工程
背景情况:
- 丝状真菌表现出多样化的潜水宏观形态 (分散的,团块,颗粒) 影响产品产量和质.
- 由于复杂的生长,聚合和碎片化过程,宏观形态的发展通常是异质的.
- 对影响真菌宏观形态和异质性的培养参数的定量研究很少.
研究的目的:
- 为了在水下培养中建立对Aspergillus niger宏观形态的可再生控制.
- 研究种植参数对真菌颗粒形态和异质性的定量影响.
- 为了将颗粒的特性与蛋白质生产和葡萄糖消耗相关联.
主要方法:
- 开发了摇瓶种植条件,其中包括多种子数量,粉度,摇频率和形 (48个条件).
- 利用高通量2D图像分析量化宏观形态 (颗粒直径) 和异质性.
- 采用微CT (μ-CT) 来分析在细胞水平上真菌颗粒的3D内部结构.
主要成果:
- 确定了最小的宏观形态变化在瓶内和瓶间的最佳条件.
- 证明了颗粒直径的可重复调整,将较大的颗粒与增加的蛋白质分泌和减少的葡萄糖消耗相关联.
- 已建立的线性回归模型识别了子度,动频率和滑石度作为影响颗粒直径的关键参数.
- 基于内部子核心结构 (单子,多子,聚合成熟颗粒) 的分类颗粒使用μ-CT.
结论:
- 开发了简单的方法来控制真菌的宏观形态和异质性,以促进产品标题的优化.
- 提供了关于培养参数如何影响颗粒内部架构的第一个调查.
- 提出了基于内部子核心架构的新型颗粒分类系统,进步了对真菌发育的理解,并使生物工艺工程成为可能.
关键词:
黑色阿斯珀吉勒斯 (Aspergillus niger) 是一个的直径分布.异质性 异质性 异质性宏观形态学 宏观形态学微观形态学 微观形态学颗粒颗粒颗粒是什么意思种子培养文化种子培养文化石微粒是一种石微粒.更多相关视频
11:30Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software
Published on: July 24, 2021
3.7K
10:10Mass Production of Entomopathogenic Fungi, Metarhizium robertsii and Metarhizium pinghaense, for Commercial Application Against Insect Pests
Published on: March 31, 2022
7.0K
相关概念视频
01:18Bioreactor Controls-II
61
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
61
01:22Bioreactor Controls-III
55
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
55
01:30Designing Growth Media for Bioreactors
67
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
67
01:14Scale-Up Processes
89
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
89