相关实验视频

Updated: Jun 16, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

RNA测序确定了WT1过度表达是急性髓性白血病中不良结果的预测因素

Harsh Goel¹, Avanish Kumar Pandey¹, Rahul Kumar¹

¹Laboratory Oncology Unit, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi 110029, India.

|June 13, 2025

概括

在诊断时急性髓性白血病 (AML) 中,威尔姆斯瘤1 (WT1) 基因过度表达预测生存率不佳. 随着治疗,WT1的水平会下降,这使得它成为预后和指导治疗决策的宝贵生物标志物.

科学领域:

血液学血液学血液学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

急性髓性白血病 (AML) 是一种复杂的血液癌症,复发率高.
威尔姆斯瘤1 (WT1) 基因在AML病原和临床结果中的作用需要进一步阐明.
了解WT1基因表达对于改善AML患者管理至关重要.

研究的目的:

通过使用RNA测序来研究新型AML中的WT1基因表达动态.
评估WT1表达在AML诊断,预后和治疗反应中的临床相关性.
评估WT1作为风险分层和AML治疗指南的潜在生物标志物.

主要方法:

预期采集345个诊断,259个诱导后和70个复发期AML样本.
在配对的诊断-复发样本上进行RNA测序,以描述转录组变化和WT1表达.
定量PCR (qPCR) 用于WT1验证和Cox回归分析用于预后影响评估.

主要成果:

在AML患者的诊断时,WT1基因显著过度表达,在诱导后疗法下降.
高诊断性WT1表达与不良的临床特征相关 (增加WBC,更高的爆发百分比).
证明WT1表达是显著的预后标记,与整体存活率 (OS) 和无事件存活率 (EFS) 相关.

结论:

WT1表达是AML的关键生物标志物,反映了诊断,预后和治疗反应.
WT1水平可以帮助监测疾病状况和风险分层.
这些发现支持WT1在指导AML治疗决策和开发向疗法的潜在作用.

关键词:

在WT1中,WT1就是WT1.急性骨髓性白血病 (AML) 是一种急性骨髓性白血病.生物标志物生物标志物基因表达的基因表达方式预后标志物是一个预后标志物.

更多相关视频

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

相关概念视频

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...