相关实验视频

Updated: Jun 16, 2025

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Published on: September 18, 2020

红斑肠膜通过诱导M1巨细胞极化来调节免疫反应

Anna Bircher¹, Egle Katkeviciute¹, Yasser Morsy¹

¹Department of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.

International journal of molecular sciences

|June 13, 2025

概括

一种有益的肠道细菌Roseburia intestinalis通过激活免疫系统,显示出对抗结直肠癌 (CRC) 的潜力. 研究表明,它将免疫细胞转移到抗瘤M1表型,为CRC治疗提供新的治疗途径.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
在瘤学瘤学.

背景情况:

肠道微生物组在癌症的发展中发挥着作用,在结直肠癌 (CRC) 中观察到不平衡.
像Roseburia intestinalis这样的有益细菌在CRC患者中减少.
以前的研究表明,R. intestinalis在小鼠中具有抗瘤作用.

研究的目的:

研究R.intestinalis在结直肠癌中的分子机制.
突出R. intestinalis在CRC中的潜在治疗价值.

主要方法:

在体外共同培养实验中,对巨细胞和癌细胞进行了实验.
对巨细胞表型 (M1/M2) 和基因表达的分析.
测量化学激素和细胞激素分泌.

主要成果:

暴露于R. intestinalis增加了M1巨细胞表型,并减少了M2表型.
巨细胞中的基因表达特征类似于M1激活.
在癌细胞中观察到明显的化学激素和细胞激素分泌,这表明免疫激活.
没有观察到对癌细胞的直接细胞毒性影响.

结论:

肠道菌具有巨分极性质,促进免疫激活环境.
这种细菌通过调节免疫反应,显示出在结直肠癌中具有治疗用途的潜力.
需要进一步的研究来探索R. intestinalis对CRC病原和治疗的影响.

关键词:

这种病原体是Peptostreptococcus stomatis.罗斯布里亚肠道病 (Roseburia intestinalis) 是一种肠道病.结肠直肠癌是什么意思巨细胞的两极分化我们的微生物群.

更多相关视频

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Published on: May 21, 2012

相关概念视频

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...