新型circRNA803/lncRNA MSTRG.19726-oar-let-7a-CPEB1 ceRNA网络的定向关系的验证,基于全转录组测序的单产和多产羊的卵泡发育的关键
1College of Life Science, Jilin Normal University, Siping 136000, China.
International journal of molecular sciences
|June 13, 2025
概括
这项研究揭示了一种新的竞争性内源RNA (ceRNA) 网络,circRNA803/lncRNA MSTRG.19726-oar-let-7a-CPEB1,对于调节卵泡主导和增强绵羊生殖能力至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 非编码RNA (ncRNA) 规则
背景情况:
- 了解控制卵巢发育和绵羊生殖能力的分子机制对于提高畜牧业效率至关重要.
- 非编码RNAs (ncRNAs) 在基因调节中发挥关键作用,但它们在绵羊卵巢发育和 litter 尺寸特征中的具体参与仍未得到充分探索.
研究的目的:
- 研究多羊卵巢发育过程中ncRNAs的分子调节机制.
- 确定提高绵羊生殖能力的关键调节基因和ncRNA,重点关注毛囊主导.
主要方法:
- 在小尾汉 (多) 和Ujumuqin (单) 绵羊卵巢组织中对ncRNA和mRNA表达特征进行比较分析.
- 差异表达的 (DE) lncRNAs,circRNAs,miRNAs和mRNAs的识别.
- 使用双露西法酶记者测试,构建和功能验证竞争的内源RNA (ceRNA) 调控网络.
主要成果:
- 确定了411个DE lncRNAs,322个DE circRNAs,26个DE miRNAs和29个DEGs.
- 丰富分析揭示了DE基因参与细胞脱差,胚胎发育和关键信号通路 (例如,Hippo).
- 一个新的ceRNA网络,circRNA803/lncRNA MSTRG.19726-oar-let-7a-CPEB1被确定,证明了oar-let-7a在通过CPEB1调节毛囊主导地位方面的作用.
结论:
- 已识别的ceRNA网络,circRNA803/lncRNA MSTRG.19726-oar-let-7a-CPEB1,在调节绵羊的毛囊主导方面发挥着至关重要的作用.
- 这些发现提供了关于绵羊生殖潜力的基本见解,并提供了提高生殖效率的策略.
- 欧尔-莱特-7a成为一个关键的miRNA,通过其在已识别的ceRNA网络中的相互作用来调节多个后代特征.
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