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银纳米粒子作为一种新型组织防腐剂:与10%中性缓冲形式素进行比较研究

Safa Taha¹, Amina Ismaeel², Muna Aljishi¹

¹Department of Molecular Medicine, College of Medicine and Health Sciences, Princess Al Jawhara Center for Molecular Medicine, Genetics and Inherited Diseases, Arabian Gulf University, Manama 329, Bahrain.

International journal of molecular sciences

|June 13, 2025

概括

银纳米粒子 (AgNPs) 与传统的甲固定相比,在组织中对DNA,RNA和蛋白质提供了更好的保存. 虽然甲素更好地保留了形态,但AgNP显示为分子分析的替代品.

科学领域:

生物材料科学生物材料科学
分子生物学分子生物学
组织病理学组织病理学

背景情况:

传统的组织保护方法,如甲固定,存在挑战,包括长期损害组织完整性和潜在的生态毒性.
银纳米粒子 (AgNPs) 具有抗菌性质,使其成为新型组织保护应用的候选者.

研究的目的:

评估银纳米颗粒 (AgNPs) 作为10%中性缓冲甲 (NBF) 的潜在替代组织固定剂.
为了比较AgNPs和NBF在维护分子完整性 (DNA,RNA,蛋白质) 和组织形态方面的有效性.

主要方法:

使用化学还原方法合成AgNP.
用10%的NBF或AgNPs溶液保存了72小时的小鼠心脏,肝脏和脏组织.
通过量化核酸完整性,评估蛋白质稳定性和进行组织病理学检查来评估保存.

主要成果:

在72小时内,AgNPs溶液在整个组织中保持了一致的DNA,RNA和蛋白质质量和质量.
在同一时期,形式素治疗导致分子降解.
与AgNP相比,10%的NBF显示组织形态的保存优于AgNP.

结论:

AgNPs对核酸和细胞内蛋白质具有很好的保存性,这表明它有可能作为分子测试中的甲替代品.
对于保存组织形态而言,形式素固定仍然优越.
需要进一步的研究来优化AgNP,以改善组织样本中的形态保存.

关键词:

10%的中性缓冲甲 (10%的NBF)在BALB/c小鼠中.在GAPDH中使用.这是RT-PCR.西方黑斑是因为西方黑斑.核酸提取核酸提取蛋白质提取蛋白质提取银纳米粒子 (AgNP) 是一种

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