相关实验视频
Updated: Jun 16, 2025
11:13
Mouse Adipose Tissue Collection and Processing for RNA Analysis
Published on: January 31, 2018
48.6K
编码和非编码RNA在不同养系统下的雅克脂肪沉积中的相关性
Lin Xiong1,2, Jie Pei1,2, Shaoke Guo1,2
1Key Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
International journal of molecular sciences
|June 13, 2025
概括
这项研究揭示了雅克脂肪沉积的关键RNA调节器,确定了PPAR和PI3K-Akt等关键信号通路. 这些发现支持雅克的繁殖和生产改进.
科学领域:
- 牲畜遗传学和基因组学
- 脂肪代谢的分子生物学
- 在动物科学中的转录学.
背景情况:
- 脂肪沉积对青海-西藏高原鱼的生存和新陈代谢至关重要.
- 编码和非编码RNA (ncRNA) 都是已知的动物脂肪沉积的调节者.
研究的目的:
- 研究各种RNA类型 (mRNAs, lncRNAs, miRNAs, circRNAs) 在雅克皮下脂肪沉积中的作用.
- 确定关键的调节RNA和参与雅克脂肪代谢的信号通路.
主要方法:
- 在放牧和食条件下,雅克皮下脂肪的全转录组测序.
- 对mRNAs,lncRNAs,miRNAs和circRNAs的差异表达分析.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
- 同表达RNA (ceRNA) 网络的构建.
主要成果:
- 鉴定了677种差异表达的mRNA,120种DE lncRNA,2216种DE circRNA和15种DE miRNA.
- 发现雅克脂肪沉积主要由PPAR,PI3K-Akt和cAMP信号通路调节.
- 突出了特定的监管途径,包括TCONS00042948,TCONS00012083/bta-miR-2316/MCAT和NR4A3,作为潜在的关键.
结论:
- 这项研究阐明了复杂的RNA调节网络,该网络控制着雅克的脂肪沉积.
- 确定了关键的分子参与者和途径,这些途径可以作为改善克生产的目标.
- 为基因选择和养计划提供理论基础,旨在提高鱼肉质量和产量.
相关概念视频
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
02:05Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K
01:30Regulation of Food Intake
205
Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
205