殖民细菌在流感期间加剧炎症,细胞毒性和免疫防御 流感病毒感染
Liane Giebeler1, Christina Ehrhardt1, Antje Häder2
1Section of Experimental Virology, Institute of Medical Microbiology, Center for Molecular Biomedicine (CMB), Jena University Hospital, 07745 Jena, Germany.
像Staphylococcus epidermidis这样的共生细菌不会对流感A病毒进行保护,但可能会训练免疫细胞. 黄金葡萄球菌在共感染期间恶化肺损伤.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 呼吸道是各种各样的细菌的宿主,包括开始性和病原体.
- 了解细菌与病毒感染的相互作用对于呼吸系统健康至关重要.
研究的目的:
- 用细胞培养模型研究细菌殖民对流感A病毒 (IAV) 感染的影响.
- 为了确定共生细菌 (Staphylococcus epidermidis,Streptococcus salivarius) 和病原体 (Staphylococcus aureus) 在IAV病变发生中的作用.
主要方法:
- 在IAV感染之前,呼吸道上皮细胞被活体或无活体细菌殖民.
- 评估了细胞完整性,细胞毒性 (LDH测定) 和炎症性细胞因子表达.
- 评估巨细胞功能,以应对细菌和病毒的挑战.
主要成果:
- 起初,共生菌保持了上皮完整性,而金黄色细菌造成了显著的损伤.
- 黄金菌与IAV的同时感染导致严重的上皮细胞破坏.
- 康曼萨尔增强了炎症,并没有保护IAV,其作用取决于活细菌.
- 之前接触到共生动物改善了巨细胞对IAV的反应.
结论:
- 连体细菌不会直接保护上皮细胞免受IAV的侵害,但可以增强炎症.
- 活细菌对于观察到的效果至关重要.
- 交叉体可能有助于免疫训练,可能增强肺部防御机制对病毒感染.
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