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刺在精子生成中的信号功能和在Eriocheir sinensis中保持血淋巴-Testis屏障的稳定性

Jun-Jie Yu¹, Hong-Yu Qi¹, Zhan Zhao¹

¹The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.

International journal of molecular sciences

|June 13, 2025

概括

(HH) 信号干扰在中国小的丸中损害了血淋巴-丸屏障和生殖细胞周期. 这项研究揭示了HH信号传递.

科学领域:

发展生物学发展生物学
细胞生物学细胞生物学
生殖生物学生殖生物学

背景情况:

刺 (HH) 信号传递对各种系统的发育至关重要,包括神经系统,骨,软骨和丸.
虽然HH信号在丸中的作用在哺乳动物中得到了研究,但它与丸功能中的细胞结节的联系仍未得到充分研究.
之前关于HH信号传递和细胞结合的研究主要集中在神经系统和肠道上.

研究的目的:

在 *Eriocheir sinensis* 丸中识别HH信号通路成员.
研究HH信号在调节血淋巴-丸屏障 (HTB) 和胚胎细胞循环中的作用.
探索HH信号传递和*Eriocheir sinensis*丸中的细胞结合之间的关系.

主要方法:

在E. sinensis*丸中识别和表达HH信号组件 (HH,Smoothen,Patched,Kif27,Ci) 的分析.
Knockdown 的 *HH* 基因观察对丸结构和 HTB 完整性的影响.
生物-斯特雷普塔维丁免疫光,西部斑点和免疫光试验分析蛋白质水平,局部和细胞结构.

主要成果:

确定并表达在 *E. sinensis* 生殖细胞中的 HH 信号元件.
*HH*的淘汰导致间歇性空间扩大,破坏了HTB,降低了皮宁,HH信号,增殖和亡蛋白的水平.
观察到结位蛋白的脱位,F-actin异常,以及生殖细胞增殖和细胞亡的减缓;Wnt-β-catenin信号传递没有被激活.

结论:

HH信号传递在维持 *E. sinensis* 丸中的血淋巴 - 丸屏障完整性方面发挥着重要作用.
HH信号参与调节生殖细胞周期,影响增殖和亡.
这项研究突出了HH信号在丸生理学中的新功能,超出了目前的理解.

关键词:

这里是Eriocheir sinensis的原点.细胞结节细胞结节刺是一种刺,刺是一种刺.血淋巴试管体的障碍物阻碍物精子的产生是精子的产生.试管婴儿试管婴儿试管婴儿试管婴儿试管婴儿

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