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Linzhi Fang¹, Rong Su¹, Cunyan Li¹

¹Key Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Plants (Basel, Switzerland)

|June 13, 2025

概括

米增强疾病易感性1 (EDS1) 和植物素缺乏4 (PAD4) 蛋白质对于植物防御至关重要. 这些基因的突变通过影响防御激素水平,增加了大米对棕色植物 (BPH) 的易感性.

科学领域:

植物病理学植物病理学
分子植物-昆虫相互作用
植物生物化学植物生物化学

背景情况:

增强疾病易感性1 (EDS1) 和植物素缺陷4 (PAD4) 在植物免疫中的作用在Arabidopsis*中被确立. 它们在米中的功能 (*Oryza sativa*) 抵抗棕色虫 (*Nilaparvata lugens* Stål),这是一个主要的米害虫,尚未被探索.
棕色虫 (BPH) 在全球范围内对大米作物造成重大破坏,需要对抗药机制进行研究.

研究的目的:

研究大米EDS1 (OsEDS1) 和大米PAD4 (OsPAD4) 在抗BPH的作用.
阐明酸 (SA) 和酸 (JA) 在OsEDS1和OsPAD4介导的BPH耐药性中信号通路的参与.

主要方法:

在BPH感染后对*OsEDS1*和*OsPAD4*转录水平的分析.
在BPH挑战下对*eds1*和*pad4*突变大米系的表型分析,包括蜂蜜露分泌和宿主偏好测定.
在正常和BPH感染条件下,量化SA和JA水平,并分析野生类型和突变植物中与防御相关的基因的表达.

主要成果:

BPH 感染迅速改变了 * OsEDS1 * 和 * OsPAD4 * 转录水平.
*OsPAD4*或*OsEDS1*的突变显著增加了大米对BPH的敏感性,由更高的蜂蜜露产量和BPH宿主偏好证明.
在*OsEDS1*或*OsPAD4*中功能丧失导致基础SA和JA水平降低,并抑制SA/JA生物合成和信号基因的表达,影响防御反应.

结论:

OsPAD4和OsEDS1积极调节了对棕色虫的抗米能力.
这些蛋白质对于维持SA和JA的基底水平以及在BPH攻击时适当诱导防御通路至关重要.

关键词:

这是一种Oryza sativa.在OSEDS1中.在 OsPAD4 中使用.棕色的植物跳

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