黄色激情果的酒精发酵过程中Saccharomyces cerevisiaeE1菌株的定性蛋白质分析:第一个近似值
Roger Consuegra-Rivera1,2,3, Juan J Román-Camacho1, Inés M Santos-Dueñas2
1Department of Agricultural Chemistry, Edaphology and Microbiology, Microbiology Area, Agrifood University of Córdoba, Campus of International Excellence ceiA3, 14014 Cordoba, Spain.
Foods (Basel, Switzerland)
|June 13, 2025
概括
这项研究分析了黄色激情果酒发酵过程中的酵母蛋白. 确定了关键蛋白质,提供了改善热带水果酒和感官素质的见解.
科学领域:
- * 葡萄酒学和发酵科学
- * 蛋白质组学和酵母生物学
背景情况:
- * 黄色的激情果是葡萄酒制作的可行的基质,因为它的感官和营养价值.
- * 了解酵母蛋白质组概况对于优化发酵和葡萄酒感官特性至关重要.
研究的目的:
- * 在黄色激情果发酵过程中分析Saccharomyces cerevisiae菌株E1的蛋白质组概况.
- *确定参与发酵过程的关键酵母蛋白及其功能作用.
主要方法:
- * 在5L生物反应器中控制发酵黄色的激情果泥和糖糖.
- *在4天内监测酒精含量和糖分的下降.
- * 液体染色学-并联质谱学 (LC/MS-MS) 用于蛋白质的识别和分析.
主要成果:
- * 共有938种蛋白质被确定 (454种来自水果,484种来自酵母).
- * 243种酵母蛋白在整个发酵过程中始终存在.
- *基因本体学 (GO) 分析揭示了在分子结合,催化活性,细胞和代谢过程中的主导作用.
结论:
- * 这项研究为热带水果果中Saccharomyces cerevisiae的蛋白质动力学提供了重要的见解.
- *已识别的蛋白质为优化激情果葡萄酒生产和增强器官质量提供了基础.
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