从沙拉米和软奶酪中分离出来的Citrobacter braakii:一个新兴的食品安全危险?
Frédérique Pasquali1, Cecilia Crippa1, Alex Lucchi1
1Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna, 40127 Bologna, Italy.
Foods (Basel, Switzerland)
|June 13, 2025
概括
在手工食品中发现的Citrobacter braakii可能会对食品安全构成威胁. 与临床分离物密切相关的菌株携带抗生素耐药性基因,并在昆虫模型中引起高死亡率.
科学领域:
- 食品微生物学 食品微生物学
- 基因组学就是基因组学.
- 发现病原体发现
背景情况:
- 菌 (Citrobacter braakii) 殖民于人类和动物,作为一种机会性病原体.
- 它作为一种食源性病原体的作用是不确定的,尽管它与食物和环境来源隔离.
研究的目的:
- 评估来自手工食品生产的Citrobacter braakii对食品安全的潜在危害.
- 为了比较来自食物的C. braakii基因组与临床分离物,并评估病原性.
主要方法:
- 基因组测序和食品衍生的C. braakii与公共基因组的比较.
- 基因组学分析以确定与临床菌株的遗传关系.
- 在体内使用Galleria mellonella幼虫进行致病性测试.
主要成果:
- 来自食物的C. braakii分离物与临床C. braakii菌株具有密切的基因组相似性.
- 所有分离物都携带了blaCMY和/或qnrB基因,从而产生抗生素耐药性.
- 对vex和tvi操作子 (Vi抗原) 阳性菌株导致G. mellonella90%的死亡率,与缺乏这些操作子 (40%的死亡率) 的菌株不同.
结论:
- 来自手工食品生产的Citrobacter braakii可能是一个重要的食品安全危险.
- 基因组和病原性数据支持C. braakii作为一种食源性病原体的潜力,特别是具有特定毒性因子的菌株.
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