从Schizochytrium limacinum中通过超声波辅助提取多糖类,使用欧性溶剂的食:结构特性和抗氧化活性
Xinyu Li1, Jiaxian Wang1, Guangrong Huang1
1College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Foods (Basel, Switzerland)
|June 13, 2025
概括
这项研究优化了从Schizochytrium limacinum粉中提取多糖的超声波提取,达到9.25%的高产量. 提取的多糖体 (SLMP) 显示出与其结构和成分相关的显著抗氧化活性.
科学领域:
- 海洋生物技术 海洋生物技术
- 食品科学 食品科学 食品科学
- 生物化学 生化学
背景情况:
- 尽管含有具有潜在应用的多糖体,但Schizochytrium limacinum粉没有得到充分利用.
- 来自Schizochytrium limacinum粉 (SLMPs) 的多糖类需要结构阐明才能理解它们的活性.
- 系统地调查SLMP的结构-活动关系对于其发展至关重要.
研究的目的:
- 为了优化SLMP的提取,使用超声波辅助的以溶剂为基础的方法.
- 描述SLMP的结构,并将其与其抗氧化活性相关联.
- 建立具有保存生物活性的SLMP的高效提取协议.
主要方法:
- 使用响应表面方法 (RSM) 来优化超声波提取参数.
- 用FTIR,NMR和HPGPC分析净化SLMP的结构特征.
- 评估了抗氧化活性,并与分子量,单糖组成和SEM衍生结构特征相关.
主要成果:
- 优化的提取条件产生了9.25%的SLMP,明显高于传统方法 (4.18%).
- SLMPs含有葡萄糖,银河糖和阿拉比诺糖,其中较低的分子量分数显示出增强的抗氧化活性.
- SEM揭示了SLMPs中的多孔结构,能够合氧化还原活性功能组.
结论:
- 超声波辅助的以欧性溶剂为基础的提取是一种有效的方法,用于获得具有高产量和保存抗氧化特性的SLMP.
- 该研究为了解SLMPs的结构-活动关系提供了基础,使其能够在未来使用.
- 优化的提取条件和表征数据支持在各种应用中开发SLMP.
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