评估被转质胺酶结合甘化物修改的花生粉的功能和结构性质
Yan Liu1, Tingwei Zhu1, Fusheng Chen1
1College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China.
Foods (Basel, Switzerland)
|June 13, 2025
概括
花生粉 (PM) 经过转谷氨酸酶 (TGase) 合糖化改造,显著改善了其乳化,发泡和油吸收能力,用于增强食品应用.
科学领域:
- 食品科学与技术 食品科学与技术
- 蛋白质化学 蛋白质化学
- 生物化学工程 生物化学工程
背景情况:
- 花生粉 (PM) 是一种富含蛋白质的副产品,具有食品应用的潜力.
- 提高PM的功能性质对于增加其附加值和扩大其在食品加工中的使用至关重要.
- 转氨酸酶 (TGase) 结合糖化是一种修改蛋白质功能的一种新方法.
研究的目的:
- 为了研究冷压花生粉 (PM) 的修改,使用转质胺酶 (TGase) 结合的糖化.
- 提高PM的功能性质,包括乳化,发泡和吸油.
- 评估修改条件对修改后的PM的结构和功能特征的影响.
主要方法:
- 冷压花生粉 (PM) 经过转谷氨酸酶 (TGase) 结合糖化改性.
- 修改条件的优化:PM度,PM/葡萄糖质量比,温度和时间.
- 使用水接触角度,光光谱学和里埃变换红外光谱学评估结构性质.
- 功能性质的评估:乳化活性,发泡能力和吸油能力.
主要成果:
- 结合TGase的糖化改变了PM的二次结构,增加了水接触角度和表面的疏水性.
- 修改后的PM表现出显著增强的乳化,发泡和吸油性能.
- 乳化活性增加了69.8%,发泡能力增加了84.00%,油吸收能力增加了359.57%.
结论:
- 结合TGase的糖化是一种有效的方法来增强花生粉的功能性质.
- 改性花生粉 (EPM,FPM,OPM) 在乳化,发泡和油吸收方面表现出卓越的性能.
- 这种修改策略有望增加花生粉的附加值并扩大花生粉的食品加工应用.
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