装有多功能基金属有机框架的三维生物打印支架 改善衰老微环境 促进老年骨缺陷修复
Xin Sun1,2, Xiang Xu1,3, Xue Zhao4
1Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai 200011, China.
ACS nano
|June 13, 2025
概括
这项研究开发了新的3D生物打印支架,使用和金属有机框架来对抗氧化应激和炎症,通过延迟干细胞衰老,有效地促进老老鼠的骨再生.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 与年龄相关的骨缺陷是一个重大的健康问题,通常由衰老的微环境 (SME) 加剧,损害骨髓中介质干细胞 (BMSC) 功能和骨再生.
- 高氧化应激和SME中的炎症有助于BMSC衰老,阻碍老年人有效修复骨.
研究的目的:
- 用 (Mg) 和 (Ce) 离子为基础的金属有机框架 (MOFs) 设计多功能3D生物打印支架.
- 调查这些支架的潜力,以清理反应性氧物种 (ROS),释放Mg2+,并减轻SME,以改善与年龄相关的骨缺陷修复.
- 评估结合Wnt/β-catenin激动剂 (SKL2001) 的协同效应,以进一步增强再生能力.
主要方法:
- 通过热水方法制造基于Mg-Ce-MOF的多功能3D生物打印支架.
- 在氧化应激下对BMSC衰老和M2巨细胞两极分化的支架效应的体外评估.
- 在体内评估脚手架的有效性,促进修复临界大小的形缺陷在老鼠模型.
主要成果:
- -Ce-MOF支架有效地清除了ROS,并可持续地释放了Mg2+,延迟了BMSC衰老,并促进了M2巨细胞的两极分化.
- 释放的Mg2+激活了Nrf2信号通路,增加了阿尔德海德脱酶3A1的表达,并进一步抑制了BMSC衰老.
- 添加SKL2001显著增强了再生效果,复合脚手架加速了老老鼠的骨缺陷修复.
结论:
- 基于Mg-Ce-MOF和SKL2001的多功能3D生物打印支架是改善衰老的微环境的有希望的策略.
- 这些支架有效地延迟了BMSC衰老,并促进了骨质分化,为与年龄相关的骨缺陷修复提供了一种可行的治疗方法.
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