同时双色刺激拉曼散射生物分子成像与量身定制的超连续生成和相位敏感检测.
Le Xin1, Zhiwei Huang1,2,3
1Optical Bioimaging Laboratory, Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore, 117576, Singapore.
Small methods
|June 13, 2025
概括
我们开发了双色刺激拉曼散射 (DC-SRS) 显微镜,用于同时对细胞振动进行成像. 这种先进的技术揭示了新陈代谢动态和化合物定位在活细胞和瘤球体.
科学领域:
- 生物医学光学 生物医学光学
- 细胞成像 细胞成像
- 频谱学是一种光谱学.
背景情况:
- 刺激拉曼散射 (SRS) 显微镜可实现无标签的振动成像.
- 同时对多个拉曼波段进行成像对于复杂的生物样本至关重要.
- 当前的SRS技术在同时进行多频段检测时经常面临局限性.
研究的目的:
- 开发一种新的双色SRS (DC-SRS) 显微镜系统.
- 在高波数和细胞静音光谱区域实现同时拉曼成像.
- 为了证明DC-SRS对活细胞和组织分析的实用性.
主要方法:
- 从光子晶体纤维中利用超连续生成,产生双束 (800和850nm).
- 在斯托克斯光束 (1040 nm) 上采用相位移方案,用于单光二极管检测.
- 集成了一个双通道锁定放大器,用于同时获取信号.
主要成果:
- 成功证明了同时对C-H (2935厘米-1) 和C-D/CC (2110/2216厘米-1) 拉曼波段进行SRS成像.
- 揭示了细胞下代谢动力学和活细胞中精确定位基/基标记化合物.
- 展示了瘤球体中细胞代谢和药物细胞相互作用的快速定量监测.
结论:
- 开发的DC-SRS显微镜能够同时进行高灵敏度的多频段拉曼成像.
- 这种技术在生物和生物医学研究中推进了高分辨率的代谢功能成像.
- 在复杂模型中,DC-SRS具有研究细胞代谢和药物相互作用的巨大潜力.
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