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Updated: Jun 14, 2025

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
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单个活细胞中蛋白质的时空分析:方法和应用

Yanrong Wen1, Fenxia Lin2, Zhen Liu3

  • 1Nanjing Drum Tower Hospital: Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Department of Nuclear Medicine, CHINA.

Chembiochem : a European journal of chemical biology
|June 13, 2025
PubMed
概括

在活细胞中检测蛋白质的位置和时间是很困难的. 本综述涵盖了单细胞空间时间蛋白质分析的新方法,有助于细胞生物学和诊断.

关键词:
细胞过程是细胞的过程.活细胞 活细胞 活细胞蛋白质的功能 蛋白质的功能一个单细胞分析.时间空间的动态.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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